Literature summary for 4.2.1.30 extracted from

Wang, X.D.; Zhang, X.E.; Guo, Y.C.; Zhang, Z.P.; Cao, Z.A.; Zhou, Y.F.
Characterization of glycerol dehydratase expressed by fusing its alpha- and beta-subunits (2009), Biotechnol. Lett., 31, 711-717.

Activating Compound

Activating Compound	Comment	Organism	Structure
adenosylcobalamin	-	Klebsiella pneumoniae
ATP	-	Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment)	Organism
PCR products of gdh genes for co-expressing alpha-, beta- and gamma subunits of wild-type GDH or GDHA/CB and GDHAB/C fusion constructs inserted into pET32a vector using NdeI/HindIII sites. To minimize the effect of steric hindrance, a linker peptide inserted into the fusion partners by SacI sites, resulting two plasmids (pET32aGDHA/CLB and pET32aGDHALB/C) for expressing fusion enzyme GDHA/CLB and GDHALB/C, expressed in Escherichia coli AD494(DE3)	Klebsiella pneumoniae

Cloned (Comment)

Organism

PCR products of gdh genes for co-expressing alpha-, beta- and gamma subunits of wild-type GDH or GDHA/CB and GDHAB/C fusion constructs inserted into pET32a vector using NdeI/HindIII sites. To minimize the effect of steric hindrance, a linker peptide inserted into the fusion partners by SacI sites, resulting two plasmids (pET32aGDHA/CLB and pET32aGDHALB/C) for expressing fusion enzyme GDHA/CLB and GDHALB/C, expressed in Escherichia coli AD494(DE3)

Klebsiella pneumoniae

Inhibitors

Inhibitors	Comment	Organism	Structure
glycerol	similar to the wild-type enzyme, GDHAB/C and GDHALB/C undergo mechanism-based inactivation by glycerol during catalysis and can be reactivated by glycerol dehydratase reactivase (GDHR), adenosylcobalamin, ATP, and MgCl2	Klebsiella pneumoniae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17700	-	x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE	Klebsiella pneumoniae
40900	-	x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE	Klebsiella pneumoniae
42200	-	x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE	Klebsiella pneumoniae
60700	-	x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE	Klebsiella pneumoniae
83800	-	x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE	Klebsiella pneumoniae
85100	-	x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE	Klebsiella pneumoniae
161000	-	x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE	Klebsiella pneumoniae
229000	-	x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE	Klebsiella pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Klebsiella pneumoniae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by either ion exchange chromatography or on nickel chelate column	Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2-propanediol	-	Klebsiella pneumoniae	propionaldehyde + H2O	-	?
glycerol	-	Klebsiella pneumoniae	3-hydroxypropanal + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 60700 + x * 229000 + x * 161000, alphabetagamma including His6-tag, SDS-PAGE. x * 83800 + x * 17700, GDHAB/C, SDS-PAGE. x * 85100 + x * 17700, GDHALB/C, SDS-PAGE. x * 60700 + x * 40900, GDHA/CB. x * 60700 + x * 42200, GDHA/CLB, SDS-PAGE	Klebsiella pneumoniae

Synonyms

Synonyms	Comment	Organism
B12-dependent glycerol dehydratase	-	Klebsiella pneumoniae
GDH	-	Klebsiella pneumoniae

General Information

General Information	Comment	Organism
malfunction	GDH loses most of its beta-subunit after purification by either ion exchange chromatography or using a nickel chelate column	Klebsiella pneumoniae