Literature summary for 4.2.1.30 extracted from

Qi, X.; Sun, L.; Luo, Z.; Wu, J.; Meng, X.; Tang, Y.; Wei, Y.; Huang, R.
Rational design of glycerol dehydratase: Swapping the genes encoding the subunits of glycerol dehydratase to improve enzymatic properties (2006), Chin. Sci. Bull., 51, 2977-2985.

No PubMed abstract available

Search for more literature for 4.2.1.30

Application

Application	Comment	Organism
synthesis	synthesis of 1,3-propanediol	Citrobacter freundii
synthesis	synthesis of 1,3-propanediol	Klebsiella pneumoniae

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Citrobacter freundii
expression in Escherichia coli	Klebsiella pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.24	-	1,2-propanediol	-	Klebsiella pneumoniae
0.25	-	1,2-propanediol	-	Citrobacter freundii
0.59	-	glycerol	-	Citrobacter freundii
0.6	-	glycerol	-	Klebsiella pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Citrobacter freundii	A8CH84	-	-
Klebsiella pneumoniae	A8CIU9	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-nitrilotriacetate affinity chromatography followed by Sephacral S-300 gel filtration	Citrobacter freundii
Ni-nitrilotriacetate affinity chromatography followed by Sephacral S-300 gel filtration	Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
91.9	-	-	Citrobacter freundii
91.9	-	-	Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2-propandiol	-	Klebsiella pneumoniae	propionaldehyde + H2O	-	?
1,2-propanediol	-	Citrobacter freundii	propionaldehyde + H2O	-	?
glycerol	-	Citrobacter freundii	3-hydroxypropionaldehyde + H2O	-	?
glycerol	-	Klebsiella pneumoniae	3-hydroxypropionaldehyde + H2O	-	?

Subunits

Subunits	Comment	Organism
hexamer	heterohexamer of alpha2beta2gamma2	Citrobacter freundii
hexamer	heterohexamer of alpha2beta2gamma2	Klebsiella pneumoniae

Synonyms

Synonyms	Comment	Organism
glycerol dehydratase	-	Citrobacter freundii
glycerol dehydratase	-	Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Citrobacter freundii
45	-	-	Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Citrobacter freundii
8.5	-	-	Klebsiella pneumoniae

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Release 2023.1 (January 2023)