Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Li+ | inactivates subunit B | Klebsiella pneumoniae | |
Na+ | inactivates subunit B | Klebsiella pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stabilizes subunit A | Klebsiella pneumoniae | |
Na+ | 2 M NaCl, stabilizes subunit A | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol | Klebsiella pneumoniae | - |
? | - |
? | |
glycerol | Klebsiella pneumoniae PZH 572 Warsaw | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Klebsiella pneumoniae PZH 572 Warsaw | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol | - |
Klebsiella pneumoniae | 3-hydroxypropanal + H2O | - |
? | |
glycerol | - |
Klebsiella pneumoniae PZH 572 Warsaw | 3-hydroxypropanal + H2O | - |
? | |
glycerol | - |
Klebsiella pneumoniae | ? | - |
? | |
glycerol | - |
Klebsiella pneumoniae PZH 572 Warsaw | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | enzyme consists of two different protein subunits, A and B which associate in the presence of K+ or NH4+ forming an active apoenzyme, AB | Klebsiella pneumoniae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
studies on the thermal dissociation of the enzyme | Klebsiella pneumoniae |
50 | - |
subunit A: quickly inactivated at temperatures above, K+ and Na+ stabilize at high concentrations | Klebsiella pneumoniae |
60 | - |
dissociation of the inactive enzyme complex with hydroxocobalamin, if the enzyme complex is heated to over 60°C. Heating products: an active subunit B, an inactive subunit A, and a B12 derivative. K+ inhibit heat-mediated dissociation | Klebsiella pneumoniae |
75 | - |
subunit B is stabilized by K+, Na2HPO4 and K2HPO4 protect against thermal inactivation | Klebsiella pneumoniae |
83 | - |
3 min, 0.6 M KCl, inactive enzyme complex with hydroxocobalamin loses 50% of activity, K+ and Rb+ have the greatest heat-stabilizing effect | Klebsiella pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6-Dimethylbenzimidazolylcobamide | coenzyme required | Klebsiella pneumoniae |