Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.3 extracted from

  • Baothman, O.A.; Rolfe, M.D.; Green, J.
    Characterization of Salmonella enterica serovar Typhimurium aconitase A (2013), Microbiology, 159, 1209-1216.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
cis-aconitate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium
0.9
-
isocitrate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium
5.3
-
citrate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-aconitate + H2O
-
Salmonella enterica subsp. enterica serovar Typhimurium isocitrate
-
?
citrate
-
Salmonella enterica subsp. enterica serovar Typhimurium cis-aconitate + H2O
-
?
isocitrate
-
Salmonella enterica subsp. enterica serovar Typhimurium cis-aconitate + H2O
-
?

Subunits

Subunits Comment Organism
monomer gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms Comment Organism
Acn
-
Salmonella enterica subsp. enterica serovar Typhimurium
aconitase A
-
Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
isocitrate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium
5.2
-
cis-aconitate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium
5.3
-
citrate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

Expression

Organism Comment Expression
Salmonella enterica subsp. enterica serovar Typhimurium expression of acnA is repressed by the presence of acnB down
Salmonella enterica subsp. enterica serovar Typhimurium promoter fusion experiments show that acnA expression is regulated by the cyclic-AMP receptor protein, the fumarate nitrate reduction regulator, the ferric uptake regulator and the superoxide response protein additional information
Salmonella enterica subsp. enterica serovar Typhimurium acnA expression is enhanced in an acnB mutant up

General Information

General Information Comment Organism
malfunction acnA expression is enhanced in an acnB mutant Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1
-
citrate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium
1.2
-
isocitrate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium
26
-
cis-aconitate pH 8, 25°C, Lineweaver-Burk method Salmonella enterica subsp. enterica serovar Typhimurium