Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha, and in Bacillus subtilis strains GWH1025 and GWH1026, respectively | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
R741E/Q745E | site-directed mutagenesis, the mutant strain exhibits an increased enzymatic activity of aconitase comparing to that of the wild-type strain, because the aconitase protein expression level is significantly increased in the mutant strain | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene citB | - |
Bacillus subtilis NK1010 | - |
gene citB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | amino acid residues Arg741 and Gln745 play great role in the aconitase function | Bacillus subtilis | ? | - |
? | |
additional information | amino acid residues Arg741 and Gln745 play great role in the aconitase function | Bacillus subtilis NK1010 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aconitase | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
physiological function | aconitase is a bifunctional enzyme, which can not only interconvert citrate and isocitrate, but also has the RNA binding function similar to the eukaryotic protein IRP-1, iron regulatory protein 1 | Bacillus subtilis |