Literature summary for 4.2.1.3 extracted from

Ben-Menachem, R.; Regev-Rudzki, N.; Pines, O.
The aconitase C-terminal domain is an independent dual targeting element (2011), J. Mol. Biol., 409, 113-123.

Search for more literature for 4.2.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type enzyme and diverse pount and deletion mutants	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
C381A	site-directed mutagenesis	Saccharomyces cerevisiae
C447A	site-directed mutagenesis	Saccharomyces cerevisiae
H170A	site-directed mutagenesis	Saccharomyces cerevisiae
additional information	construction of diverse deletion mutants of aconitase, overview. Deletion of six C-terminal amino acids does not eliminate enzymatic activity but abolishes dual targeting	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	aconitase in yeast is a single translation product, which is dual targeted and distributed between the mitochondria and the cytosol by a unique mechanism involving reverse translocation, dual localization, detailed overview	Saccharomyces cerevisiae	5829	-
mitochondrion	aconitase in yeast is a single translation product, which is dual targeted and distributed between the mitochondria and the cytosol by a unique mechanism involving reverse translocation, dual localization, detailed overview	Saccharomyces cerevisiae	5739	-
additional information	subcellular localization study, overview. All mutant aconitase protein molecules are partially imported into mitochondria, then the N-terminal mitochondrial targeting sequence is cleaved off by the mitochondrial processing peptidase. After the cleavage, a subpopulation of the protein molecules moves back into the cytosol by reverse translocation. The aconitase C-terminal domain confers dual targeting	Saccharomyces cerevisiae	-	-

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-
Saccharomyces cerevisiae BY4741	-	-	-

Synonyms

Synonyms	Comment	Organism
Aco1	-	Saccharomyces cerevisiae
aconitase	-	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)