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Literature summary for 4.2.1.28 extracted from

  • Toraya, T.; Honda, S.; Mori, K.
    Coenzyme B12-dependent diol dehydratase is a potassium ion-requiring calcium metalloenzyme: evidence that the substrate-coordinated metal ion is calcium (2010), Biochemistry, 49, 7210-7217.
    View publication on PubMed
Search for more literature for 4.2.1.28

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli strain JM109 Klebsiella oxytoca

General Stability

General Stability Organism
calcium-deprived apoenzyme is unstable when incubated at 37°C in the absence of both Ca2+ and substrate Klebsiella oxytoca

Inhibitors

Inhibitors Comment Organism Structure
EDTA EDTA inhibition of apoenzyme is reversible at least in the initial phase Klebsiella oxytoca
EGTA
-
 Klebsiella oxytoca
additional information no inhibition by 5 mM of 1,10-phenanthroline, 2,2'-dipyridyl, citrate, succinate, tartrate, malate, and salicylate Klebsiella oxytoca

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1.6-1.9 mol/mol of enzyme. CaCl2 and substrate show not only a stabilizing effect but also some activating effect of about 30% Klebsiella oxytoca
Cr2+ 0.10 mol/mol of enzyme Klebsiella oxytoca
Cu2+ 0.16 mol/mol of enzyme Klebsiella oxytoca
Fe2+ 0.09 mol/mol of enzyme Klebsiella oxytoca
K+ selectively activates diol dehydratase Klebsiella oxytoca
Mg2+ 0.19-0.26 mol/mol of enzyme Klebsiella oxytoca
Mn2+ 0.09 mol/mol of enzyme Klebsiella oxytoca
additional information no requirements of divalentmetal ions for catalysis, metal contents in purified recombinant enzyme Klebsiella oxytoca
NH4+ selectively activates diol dehydratase Klebsiella oxytoca
Zn2+ 0.12 mol/mol of enzyme Klebsiella oxytoca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
207300
-
-
 Klebsiella oxytoca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
propane-1,2-diol Klebsiella oxytoca
-
 propanal + H2O
-
 ?
propane-1,2-diol Klebsiella oxytoca ATCC 8724
-
 propanal + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella oxytoca
-
-
-
Klebsiella oxytoca ATCC 8724
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of calcium-containing apoenzyme from calcium-deprived apoenzyme and Ca2+, overview Klebsiella oxytoca

Source Tissue

Source Tissue Comment Organism Textmining
additional information ATCC8724 cells grown without aeration in glycerol-1,2-propanediol medium Klebsiella oxytoca
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propane-1,2-diol
-
 Klebsiella oxytoca propanal + H2O
-
 ?
propane-1,2-diol
-
 Klebsiella oxytoca ATCC 8724 propanal + H2O
-
 ?

Synonyms

Synonyms Comment Organism
adenosylcobalamin-dependent diol dehydratase
-
 Klebsiella oxytoca
AdoCbl-dependent diol dehydratase
-
 Klebsiella oxytoca
coenzyme B12-dependent diol dehydratase
-
 Klebsiella oxytoca
diol dehydratase
-
 Klebsiella oxytoca
DL-1,2-propanediol hydrolyase
-
 Klebsiella oxytoca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Klebsiella oxytoca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Klebsiella oxytoca

Cofactor

Cofactor Comment Organism Structure
adenosylcobalamin i.e. coenzyme B12 or AdoCbl, dependent on Klebsiella oxytoca