Literature summary for 4.2.1.28 extracted from

Parsons, J.B.; Dinesh, S.D.; Deery, E.; Leech, H.K.; Brindley, A.A.; Heldt, D.; Frank, S.; Smales, C.M.; Luensdorf, H.; Rambach, A.; Gass, M.H.; Bleloch, A.; McClean, K.J.; Munro, A.W.; Rigby, S.E.; Warren, M.J.; Prentice, M.B.
Biochemical and structural insights into bacterial organelle form and biogenesis (2008), J. Biol. Chem., 283, 14366-14375.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene pduC, DNA and amino acid sequence determination and analysis, genetic organization in the pdu operon, overview, and recombinant enzyme expression in Escherichia coli strain JM109	Citrobacter freundii
gene pduD, DNA and amino acid sequence determination and analysis, genetic organization in the pdu operon, overview, and recombinant enzyme expression in Escherichia coli strain JM109	Citrobacter freundii
gene pduE, DNA and amino acid sequence determination and analysis, genetic organization in the pdu operon, overview, and recombinant enzyme expression in Escherichia coli strain JM109	Citrobacter freundii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	in the cobalamin cofactor	Citrobacter freundii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
propane-1,2-diol	Citrobacter freundii	-	propanal + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Citrobacter freundii	B1VB64	diol dehydratase large subunit; gene pduC is located in the pdu ooperon	-
Citrobacter freundii	B1VB65	diol dehydratase medium subunit; gene pduD is located in the pdu ooperon	-
Citrobacter freundii	B1VB66	diol dehydratase small subunit; gene pduE is located in the pdu ooperon	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
propane-1,2-diol	-	Citrobacter freundii	propanal + H2O	-	?

Subunits

Subunits	Comment	Organism
oligomer	three different subunits encoded by distinct genes	Citrobacter freundii
oligomer	three different subunits encoded by distint genes	Citrobacter freundii

Synonyms

Synonyms	Comment	Organism
diol dehydratase	-	Citrobacter freundii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Citrobacter freundii

Cofactor

Cofactor	Comment	Organism	Structure
Cobalamin	-	Citrobacter freundii

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Release 2023.1 (January 2023)