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Literature summary for 4.2.1.28 extracted from

  • Toraya, T.; Tamura, N.; Watanabe, T.; Yamanishi, M.; Hieda, N.; Mori, K.
    Mechanism-based inactivation of coenzyme B12-dependent diol dehydratase by 3-unsaturated 1,2-diols and thioglycerol (2008), J. Biochem., 144, 437-446.
    View publication on PubMed
Search for more literature for 4.2.1.28

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain JM109 Klebsiella oxytoca

Inhibitors

Inhibitors Comment Organism Structure
1,2-Butanediol
-
 Klebsiella oxytoca
3-butene-1,2-diol holodiol dehydratase undergoes rapid and irreversible inactivation, the inactivation cleaves the Co-C bond of adenosylcobalamin irreversibly forming unidentified radicals and cob(II)alamin that resist oxidation even in the presence of oxygen, inactivation mechanism, overview Klebsiella oxytoca
3-butyne-1,2-diol holodiol dehydratase undergoes rapid and irreversible inactivation, the inactivation cleaves the Co-C bond of adenosylcobalamin irreversibly forming unidentified radicals and cob(II)alamin that resist oxidation even in the presence of oxygen, inactivation mechanism, overview Klebsiella oxytoca
additional information inactivated holoenzymes undergo reactivation by diol dehydratase-reactivating factor in the presence of ATP, Mg2+ and adenosylcobalamin Klebsiella oxytoca
thioglycerol holodiol dehydratase undergoes rapid and irreversible inactivation, the inactivation cleaves the Co-C bond of adenosylcobalamin irreversibly forming unidentified radicals and cob(II)alamin that resist oxidation even in the presence of oxygen, inactivation mechanism, overview Klebsiella oxytoca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic analysis with substrate analogues 3-butene-1,2-diol, 3-butyne-1,2-diol or thioglycerol Klebsiella oxytoca
0.08
-
 1,2-propanediol pH 8.0, 37°C Klebsiella oxytoca
2.5
-
 1,2-Butanediol pH 8.0, 37°C Klebsiella oxytoca

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the is dependent on coenzyme B12, adenosylcobalamin Klebsiella oxytoca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,2-propanediol Klebsiella oxytoca
-
 propanal + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella oxytoca
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
inactivated holoenzymes undergo reactivation by diol dehydratase-reactivating factor in the presence of ATP, Mg2+ and adenosylcobalamin Klebsiella oxytoca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-butanediol
-
 Klebsiella oxytoca butanal + H2O
-
 ?
1,2-propanediol
-
 Klebsiella oxytoca propanal + H2O
-
 ?
3-butene-1,2-diol
-
 Klebsiella oxytoca ?
-
 ?
3-butyne-1,2-diol
-
 Klebsiella oxytoca ?
-
 ?
thioglycerol
-
 Klebsiella oxytoca ?
-
 ?

Synonyms

Synonyms Comment Organism
coenzyme B12-dependent diol dehydratase
-
 Klebsiella oxytoca
diol dehydratase
-
 Klebsiella oxytoca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Klebsiella oxytoca

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.8
-
 1,2-Butanediol pH 8.0, 37°C Klebsiella oxytoca
366
-
 1,2-propanediol pH 8.0, 37°C Klebsiella oxytoca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Klebsiella oxytoca

Cofactor

Cofactor Comment Organism Structure
coenzyme B12 adenosylcobalamin, dependent on, two mol of 5'-deoxyadenosine per mol of enzyme are formed as an inactivation product from the coenzyme adenosyl group in presence of inhibitors, overview Klebsiella oxytoca

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information kinetic analysis with inhibitory substrate analogues 3-butene-1,2-diol, 3-butyne-1,2-diol or thioglycerol Klebsiella oxytoca
0.02
-
 3-butyne-1,2-diol pH 8.0, 37°C Klebsiella oxytoca
0.03
-
 3-butene-1,2-diol pH 8.0, 37°C Klebsiella oxytoca
0.1
-
 thioglycerol pH 8.0, 37°C Klebsiella oxytoca
1.97
-
 1,2-Butanediol pH 8.0, 37°C Klebsiella oxytoca