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Literature summary for 4.2.1.28 extracted from
- Propanediol-1,2-dehydratase and metabolism of glycerol of Lactobacillus brevis (1984), Arch. Microbiol., 139, 366-370.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| sensitive to most solubilization procedures, dithiothreitol stabilizes | Levilactobacillus brevis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Li+ | - |
Levilactobacillus brevis |  |
| Mg2+ | - |
Levilactobacillus brevis | |
| Mn2+ | highest inhibition | Levilactobacillus brevis | |
| Na+ | - |
Levilactobacillus brevis | |
| p-chloromercuribenzoate | - |
Levilactobacillus brevis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
1,2-propanediol | - |
Levilactobacillus brevis | |
| 4 | - |
glycerol | - |
Levilactobacillus brevis | |
| 5.3 | - |
1,2-ethanediol | - |
Levilactobacillus brevis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | requirement for monovalent cation | Levilactobacillus brevis | |
| NH4+ | - |
Levilactobacillus brevis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
gel filtration | Levilactobacillus brevis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lentilactobacillus buchneri | - |
- |
- |
| Levilactobacillus brevis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Levilactobacillus brevis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, phosphate buffer, pH 7.0, at least 24 h | Levilactobacillus brevis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-ethanediol | - |
Levilactobacillus brevis | acetaldehyde | ? | ? | |
| 1,2-propanediol | - |
Levilactobacillus brevis | propanal | - |
? | |
| 1,2-propanediol | - |
Lentilactobacillus buchneri | propanal | - |
? | |
| glycerol | - |
Levilactobacillus brevis | beta-hydroxypropionaldehyde | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | - |
Levilactobacillus brevis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
50% loss of activity, 50 min | Levilactobacillus brevis |
| 60 | - |
50% loss of activity, 30 min | Levilactobacillus brevis |
| 70 | - |
50% loss of activity, 10 min | Levilactobacillus brevis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Levilactobacillus brevis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cobamide | - |
Lentilactobacillus buchneri | |
| cobamide | Km: 0.007 mM | Levilactobacillus brevis | |
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Release 2023.1 (January 2023)
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