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Literature summary for 4.2.1.28 extracted from
- Diol dehydrase (1982), B12 (Dolphin, D. ed. ) Wiley, New York, 2, 233-262.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| 10-20% glycerol stabilizes component F at 0°C | Klebsiella pneumoniae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosylcobalamin | Ki: 0.8 mM | Klebsiella pneumoniae |  |
| Chloroacetaldehyde | inactivates | Klebsiella pneumoniae | |
| EDTA | - |
Citrobacter freundii | |
| EDTA | - |
Klebsiella pneumoniae | |
| EDTA | - |
Propionibacterium freudenreichii | |
| glycerol | stereospecificity: enzyme-R-glycerol complex responsible for product-formation, enzyme-S-glycerol complex responsible for inactivation, several molecules of glycerol per enzyme molecule involved, active-site inhibition, C3-methyl group plays crucial role | Klebsiella pneumoniae | |
| glycoaldehyde | inactivates | Klebsiella pneumoniae | |
| p-chloromercuribenzoate | reversed by thiols | Klebsiella pneumoniae | |
| p-chloromercuribenzoate | - |
Propionibacterium freudenreichii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Klebsiella pneumoniae | |
| additional information | - |
additional information | - |
Citrobacter freundii | |
| additional information | - |
additional information | - |
Propionibacterium freudenreichii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cs+ | - |
Propionibacterium freudenreichii | |
| Cs+ | activates, Km: 5.0 mM | Klebsiella pneumoniae | |
| K+ | requirement for monovalent cation | Klebsiella pneumoniae | |
| K+ | requirement for monovalent cation | Citrobacter freundii | |
| K+ | requirement for monovalent cation | Propionibacterium freudenreichii | |
| K+ | Km: 0.67 mM | Klebsiella pneumoniae | |
| Na+ | - |
Propionibacterium freudenreichii | |
| Na+ | activates, Km: 19 mM | Klebsiella pneumoniae | |
| NH4+ | - |
Propionibacterium freudenreichii | |
| NH4+ | Km: 0.12 mM | Klebsiella pneumoniae | |
| Rb+ | - |
Propionibacterium freudenreichii | |
| Rb+ | Km: 1.8 mM | Klebsiella pneumoniae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Klebsiella pneumoniae |
| additional information | - |
- |
Citrobacter freundii |
| additional information | - |
- |
Propionibacterium freudenreichii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-propanediol | Klebsiella pneumoniae | - |
? | - |
? | |
| glycerol | Klebsiella pneumoniae | first step of anaerobic fermentation | ? | - |
? | |
| glycerol | Klebsiella pneumoniae | different enzyme than glycerol dehydrase | ? | - |
? | |
| additional information | Klebsiella pneumoniae | metabolic role: production of electron acceptor beta-hydroxypropionaldehyde | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
- |
- |
| Klebsiella pneumoniae | - |
formerly Aerobacter aerogenes | - |
| Propionibacterium freudenreichii | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| total inactivation after aerobic incubation, substrate and analogues protect | Klebsiella pneumoniae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| propane-1,2-diol = propanal + H2O | mechanism | Klebsiella pneumoniae | |
| propane-1,2-diol = propanal + H2O | general mechanism and stereochemistry, role of coenzyme in OH-group transfer, mechanism of overall reaction, role of free radicals during reaction, studies with coenzyme analogues | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-propanediol | - |
Klebsiella pneumoniae | propanal | - |
? | |
| 1,2-propanediol | - |
Citrobacter freundii | propanal | - |
? | |
| 1,2-propanediol | - |
Propionibacterium freudenreichii | propanal | - |
? | |
| 1,2-propanediol | - |
Klebsiella pneumoniae | ? | - |
? | |
| glycerol | first step of anaerobic fermentation | Klebsiella pneumoniae | ? | - |
? | |
| glycerol | different enzyme than glycerol dehydrase | Klebsiella pneumoniae | ? | - |
? | |
| additional information | - |
Klebsiella pneumoniae | ? | - |
? | |
| additional information | metabolic role: production of electron acceptor beta-hydroxypropionaldehyde | Klebsiella pneumoniae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | - |
Citrobacter freundii |
| More | - |
Propionibacterium freudenreichii |
| More | reassociatin of components F and S strongly promoted by substrate, component S is a sulfhydryl protein | Klebsiella pneumoniae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Klebsiella pneumoniae | |
| additional information | - |
additional information | - |
Citrobacter freundii | |
| additional information | - |
additional information | - |
Propionibacterium freudenreichii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 9.5 | - |
Klebsiella pneumoniae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cobamide | - |
Citrobacter freundii | |
| cobamide | - |
Propionibacterium freudenreichii | |
| cobamide | slow inactivation by analogues | Klebsiella pneumoniae | |
| cobamide | coenzyme serves as intermediate hydrogen carrier | Klebsiella pneumoniae | |
| cobamide | mechanism of coenzyme action | Klebsiella pneumoniae | |
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Release 2023.1 (January 2023)
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