Literature summary for 4.2.1.28 extracted from

McGee, D.E.; Richards, J.H.
Purification and subunit characterization of propanediol dehydratase, a membrane-associated enzyme (1981), Biochemistry, 20, 4293-4298.

Search for more literature for 4.2.1.28

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell membrane	associated with	Klebsiella pneumoniae	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
15000	-	1 * 60000, 1 * 51000, 1 * 29000, 1 * 15000, SDS-PAGE	Klebsiella pneumoniae
29000	-	1 * 60000, 1 * 51000, 1 * 29000, 1 * 15000, SDS-PAGE	Klebsiella pneumoniae
51000	-	1 * 60000, 1 * 51000, 1 * 29000, 1 * 15000, SDS-PAGE	Klebsiella pneumoniae
60000	-	1 * 60000, 1 * 51000, 1 * 29000, 1 * 15000, SDS-PAGE	Klebsiella pneumoniae
250000	-	gel filtration of membrane extracts	Klebsiella pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Klebsiella pneumoniae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
membrane-associated enzyme, higher yield than from cell-free extracts, 60000 subunit	Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
95	-	membrane-associated enzyme	Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1,2-propanediol	-	Klebsiella pneumoniae	propanal	-	?

Subunits

Subunits	Comment	Organism
More	N-terminal amino acid analysis, role of cysteines	Klebsiella pneumoniae
tetramer	1 * 60000, 1 * 51000, 1 * 29000, 1 * 15000, SDS-PAGE	Klebsiella pneumoniae

Cofactor

Cofactor	Comment	Organism	Structure
cobamide	-	Klebsiella pneumoniae

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Release 2023.1 (January 2023)