Cloned (Comment) | Organism |
---|---|
mutant enzymes C47A, C56A, C124A, C197A, and C441A overexpressed in Escherichia coli cells as a His6-tagged enzymes | Azospirillum brasilense |
Protein Variants | Comment | Organism |
---|---|---|
C124A | non-colored inactive mutant enzyme, the UV visible absorption spectrum contained no characteristic element of [4Fe-4S]2+ | Azospirillum brasilense |
C197A | non-colored inactive mutant enzyme, the UV visible absorption spectrum contained no characteristic element of [4Fe-4S]2+ | Azospirillum brasilense |
C441A | brown-colored mutant is significantly active and UV-visible absorption spectrum is similar to those of the wild-type enzyme | Azospirillum brasilense |
C47A | brown-colored mutant is significantly active and UV-visible absorption spectrum is similar to those of the wild-type enzyme | Azospirillum brasilense |
C56A | inactive mutant partiall shares similar behavior to the wild-type in color and UV-visible absorption spectrum | Azospirillum brasilense |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the enzyme contains a [4Fe-4S]2+ cluster | Azospirillum brasilense | |
Mg2+ | optimal concentration: 1-10 mM | Azospirillum brasilense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
2 * 62000, SDS-PAGE | Azospirillum brasilense |
128000 | - |
gel filtration | Azospirillum brasilense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azospirillum brasilense | Q1JUQ1 | ATCC 29145 | - |
Purification (Comment) | Organism |
---|---|
- |
Azospirillum brasilense |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.07 | - |
- |
Azospirillum brasilense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arabinonate | - |
Azospirillum brasilense | 2-Dehydro-3-deoxy-L-arabinonate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 62000, SDS-PAGE | Azospirillum brasilense |
Synonyms | Comment | Organism |
---|---|---|
L-Arabonate dehydratase | - |
Azospirillum brasilense |