Literature summary for 4.2.1.25 extracted from

Watanabe, S.; Shimada, N.; Tajima, K.; Kodaki, T.; Makino, K.
Identification and Characterization of L-Arabonate Dehydratase, L-2-Keto-3-deoxyarabonate Dehydratase, and L-Arabinolactonase Involved in an Alternative Pathway of L-Arabinose Metabolism: Novel Evolution ary Insight into Sugar Metabolism (2006), J. Biol. Chem., 281, 33521-33536.

Search for more literature for 4.2.1.25

Cloned(Commentary)

Cloned (Comment)	Organism
mutant enzymes C47A, C56A, C124A, C197A, and C441A overexpressed in Escherichia coli cells as a His6-tagged enzymes	Azospirillum brasilense

Protein Variants

Protein Variants	Comment	Organism
C124A	non-colored inactive mutant enzyme, the UV visible absorption spectrum contained no characteristic element of [4Fe-4S]2+	Azospirillum brasilense
C197A	non-colored inactive mutant enzyme, the UV visible absorption spectrum contained no characteristic element of [4Fe-4S]2+	Azospirillum brasilense
C441A	brown-colored mutant is significantly active and UV-visible absorption spectrum is similar to those of the wild-type enzyme	Azospirillum brasilense
C47A	brown-colored mutant is significantly active and UV-visible absorption spectrum is similar to those of the wild-type enzyme	Azospirillum brasilense
C56A	inactive mutant partiall shares similar behavior to the wild-type in color and UV-visible absorption spectrum	Azospirillum brasilense

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	the enzyme contains a [4Fe-4S]2+ cluster	Azospirillum brasilense
Mg2+	optimal concentration: 1-10 mM	Azospirillum brasilense

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
62000	-	2 * 62000, SDS-PAGE	Azospirillum brasilense
128000	-	gel filtration	Azospirillum brasilense

Organism

Organism	UniProt	Comment	Textmining
Azospirillum brasilense	Q1JUQ1	ATCC 29145	-

Purification (Commentary)

Purification (Comment)	Organism
-	Azospirillum brasilense

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.07	-	-	Azospirillum brasilense

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Arabinonate	-	Azospirillum brasilense	2-Dehydro-3-deoxy-L-arabinonate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 62000, SDS-PAGE	Azospirillum brasilense

Synonyms

Synonyms	Comment	Organism
L-Arabonate dehydratase	-	Azospirillum brasilense

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Release 2023.1 (January 2023)