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Literature summary for 4.2.1.24 extracted from
- Plastid associated porphobilinogen synthase from toxoplasma gondii: kinetic and structural properties validate therapeutic potential (2010), J. Biol. Chem., 285, 22122-22131.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant TgPBGS is expressed in Escherichia coli as a His-tagged fusion protein | Toxoplasma gondii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTA646-658 | a mutant enzyme lacking the C-terminal 13 amino acids distinguishing parasite PBGS from plant and animal enzymes is purified as a dimer, suggesting that the C-terminus is required for octamer stabilisation | Toxoplasma gondii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| succinylacetone | - |
Toxoplasma gondii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
5-aminolevulinic acid | - |
Toxoplasma gondii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| apicoplast | - |
Toxoplasma gondii | 20011 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | enzyme responds to Mg2+ but not to Zn2+, enzyme shows two Mg2+ affinities | Toxoplasma gondii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
recombinant TgPBGS also shows low levels of dimers, 2 * 40000 Da, SDS-PAGE | Toxoplasma gondii |
| 40000 | - |
recombinant TgPBGS is purified as a stable octamer, 8 * 40000 Da, SDS-PAGE | Toxoplasma gondii |
| 320000 | - |
gel filtration, recombinant TgPBGS is purified as a stable octamer | Toxoplasma gondii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Toxoplasma gondii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Toxoplasma gondii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.4 | - |
- |
Toxoplasma gondii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-aminolevulinic acid + 5-aminolevulinic acid | - |
Toxoplasma gondii | porphobilinogen + 2 H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | recombinant TgPBGS also shows low levels of dimers, 2 * 40000 Da, SDS-PAGE | Toxoplasma gondii |
| octamer | recombinant TgPBGS is purified as a stable octamer, 8 * 40000 Da, SDS-PAGE | Toxoplasma gondii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Porphobilinogen synthase | - |
Toxoplasma gondii |
| TgPBGS | - |
Toxoplasma gondii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Toxoplasma gondii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | - |
Toxoplasma gondii |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
- |
Toxoplasma gondii | succinylacetone | |
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Release 2023.1 (January 2023)
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