Crystallization (Comment) | Organism |
---|---|
sitting-drop vapour-diffusion method | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F12L | the catalytic activity is very low under conditions at which the wild-type human enzyme is most active | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the kinetic data do not follow a simple Michaelis-Menten relationship, but can be attributed to catalysis by two different forms of the enzyme that have different Km-values | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
197000 | - |
variant F12L, equilibrium sedimentation | Homo sapiens |
244000 | - |
wild-type enzyme, equilibrium sedimentation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminolevulinate + 5-aminolevulinate | Homo sapiens | the enzyme catalyzes the first common step in the biosynthesis of tetrapyrroles | porphobilinogen + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P13716 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-aminolevulinate + 5-aminolevulinate | the enzyme catalyzes the first common step in the biosynthesis of tetrapyrroles | Homo sapiens | porphobilinogen + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | wild-type enzyme and variant F12L exist in different oligomeric states. The wild-type enzyme exists as an octamer and the F12L variant exists as a hexamer. It appears that any equilibrium between octamer and hexamer most probably proceeds through the interconversion of hugging dimer and the detached dimer | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.9 | 8 | pH 5.9: about 50% of maximal activity, pH 8.0: about 70% of maximal activity, wild-type enzyme | Homo sapiens |
6.8 | - |
wild-type enzyme | Homo sapiens |