Cloned (Comment) | Organism |
---|---|
full-length CBS and truncated enzyme containing residues 1-397 are expressed in Escherichia coli BL21 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R224A | the mutation decreases CBS activity by approximately 50% | Homo sapiens |
R51A | the mutation decreases CBS activity by approximately 50% | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P35520 | - |
- |
Purification (Comment) | Organism |
---|---|
GST Trap FF column chromatography and Mono Q column chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
truncated ferrous mutant enzyme R51A, at 37°C and pH 8 | Homo sapiens |
1.3 | - |
full-length ferrous mutant enzyme R224A, at 37°C and pH 8 | Homo sapiens |
1.3 | - |
full-length ferrous mutant enzyme R51A, at 37°C and pH 8 | Homo sapiens |
2.2 | - |
full-length ferric mutant enzyme R224A, at 37°C and pH 8 | Homo sapiens |
2.2 | - |
full-length ferric mutant enzyme R51A, at 37°C and pH 8 | Homo sapiens |
2.8 | - |
full-length ferrous wild type enzyme, at 37°C and pH 8 | Homo sapiens |
3.8 | - |
truncated ferrous mutant enzyme R224A, at 37°C and pH 8 | Homo sapiens |
4 | - |
truncated ferric mutant enzyme R51A, at 37°C and pH 8 | Homo sapiens |
5.2 | - |
truncated ferric wild type enzyme, at 37°C and pH 8 | Homo sapiens |
6 | - |
truncated ferric mutant enzyme R224A, at 37°C and pH 8 | Homo sapiens |
8 | - |
truncated ferrous wild type enzyme, at 37°C and pH 8 | Homo sapiens |
10.3 | - |
full-length ferric wild type enzyme, at 37°C and pH 8 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + L-homocysteine | - |
Homo sapiens | L-cystathionine + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CBS | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the specific activity of CBS improves 1.8-2.8fold under truncation of R51A and R224A when the heme iron is in the ferric state | Homo sapiens | |
pyridoxal 5'-phosphate | - |
Homo sapiens |