Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
I278T | 1-5% of the specific activity of the wild-type enzyme. Decreased activity is due to reduced turnover rate and not substrate binding. Mutant enzyme does not have altered affinity for 5'-pyridoxal phosphate. KM-value for pyridoxal 5'-phosphate is 1.4fold lower than wild-type value. KM-value for L-serine is 1.2fold lower than wild-type value. KM-value for L-homocysteine is 3.6fold higher than wild-type value. KM-value for L-cysteine is 1.7 fold lower than wild-type value | Homo sapiens |
R266K | 30-100% of the specific activity of the wild-type enzyme. Decreased activity is due to reduced turnover rate and not substrate binding. Reduced affinity for 5'-pyridoxal phosphate compared to the wild type enzyme. KM-value for pyridoxal 5'-phosphate is 2.9fold higher than wild-type value. KM-value for L-serine is 1.6fold lower than wild-type value. KM-value for L-homocysteine is identical to wild-type value. KM-value for L-cysteine is 1.4 fold lower than wild-type value | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.41 | - |
L-serine | 37°C, pH 8.6, mutant enzyme I278T | Homo sapiens | |
1.74 | - |
L-serine | 37°C, pH 8.6, wild-type enzyme | Homo sapiens | |
2 | - |
homocysteine | 37°C, pH 8.6, mutant enzyme I278T | Homo sapiens | |
2.76 | - |
L-serine | 37°C, pH 8.6, mutant enzyme R266K | Homo sapiens | |
3.59 | - |
L-cysteine | 37°C, pH 8.6, mutant enzyme I278T | Homo sapiens | |
4.3 | - |
L-cysteine | 37°C, pH 8.6, mutant enzyme R266K | Homo sapiens | |
6.11 | - |
L-cysteine | 37°C, pH 8.6, wild-type enzyme | Homo sapiens | |
7.17 | - |
homocysteine | 37°C, pH 8.6, mutant enzyme R266K | Homo sapiens | |
7.17 | - |
homocysteine | 37°C, pH 8.6, wild-type enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + L-cysteine | - |
Homo sapiens | ? | - |
? | |
L-serine + L-homocysteine | - |
Homo sapiens | cystathionine + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | 0.51 | L-homocysteine | 37°C, pH 8.6, wild-type enzyme | Homo sapiens | |
0.04 | 1.97 | L-homocysteine | 37°C, pH 8.6, mutant enzyme R266K | Homo sapiens | |
0.04 | - |
L-cysteine | 37°C, pH 8.6, mutant enzyme I278T | Homo sapiens | |
0.052 | 2.1 | L-serine | 37°C, pH 8.6, wild-type enzyme | Homo sapiens | |
0.09 | - |
L-homocysteine | 37°C, pH 8.6, mutant enzyme I278T | Homo sapiens | |
0.15 | - |
L-serine | 37°C, pH 8.6, mutant enzyme I278T | Homo sapiens | |
1.95 | - |
L-cysteine | 37°C, pH 8.6, mutant enzyme R266K | Homo sapiens | |
2.9 | - |
L-serine | 37°C, pH 8.6, mutant enzyme R266K | Homo sapiens | |
3.13 | - |
L-cysteine | 37°C, pH 8.6, wild-type enzyme | Homo sapiens | |
4.39 | - |
L-cysteine | 37°C, pH 8.6, wild-type enzyme | Homo sapiens | |
7.93 | - |
L-homocysteine | 37°C, pH 8.6, wild-type enzyme | Homo sapiens | |
9.06 | - |
L-homocysteine | 37°C, pH 8.6, mutant enzyme R266K | Homo sapiens | |
10.19 | - |
L-serine | 37°C, pH 8.6, wild-type enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | Km-value for wild-type enzyme: 0.00118 mM, KM-value for mutant enzyme I278T: 0.00084 mM, KM-value for mutant enzyme R266K: 0.00339 mM | Homo sapiens |