Protein Variants | Comment | Organism |
---|---|---|
Q157A | no detectable beta-replacement activity, catalyzes a competing beta-elimination reaction, in which L-Ser is hydrolyzed to NH3 and pyruvate | Saccharomyces cerevisiae |
Q157E | no detectable beta-replacement activity, catalyzes a competing beta-elimination reaction, in which L-Ser is hydrolyzed to NH3 and pyruvate | Saccharomyces cerevisiae |
Q157H | enzyme suffers suicide inhibition via a mechanism in which the released aminoacrylate intermediate covalently attacks the internal aldimine of the enzyme, catalyzes a competing beta-elimination reaction, in which L-Ser is hydrolyzed to NH3 and pyruvate | Saccharomyces cerevisiae |
S82A | catalyzes a competing beta-elimination reaction, in which L-Ser is hydrolyzed to NH3 and pyruvate | Saccharomyces cerevisiae |
T81A | catalyzes a competing beta-elimination reaction, in which L-Ser is hydrolyzed to NH3 and pyruvate | Saccharomyces cerevisiae |
T85A | catalyzes a competing beta-elimination reaction, in which L-Ser is hydrolyzed to NH3 and pyruvate | Saccharomyces cerevisiae |
Y158F | 3fold decreased beta-replacement activity, enzyme suffers suicide inhibition via a mechanism in which the released aminoacrylate intermediate covalently attacks the internal aldimine of the enzyme, catalyzes a competing beta-elimination reaction, in which L-Ser is hydrolyzed to NH3 and pyruvate | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-serine + L-homocysteine = L-cystathionine + H2O | ping-pong mechanism | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + H2O | wild-type enzyme shows no beta-elimination reaction, beta elimination is only detectable in the following mutants: T81A, S82A, T85A, Q157A, Q157E, Q157H, Y158F | Saccharomyces cerevisiae | NH3 + pyruvate | - |
? | |
L-Serine + homocysteine | - |
Saccharomyces cerevisiae | Cystathionine + H2O | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.082 | - |
L-serine | Q157H mutant, pH 8.6, 37°C | Saccharomyces cerevisiae | |
0.083 | - |
L-cystathionine | S82A mutant, pH 8.6, 37°C | Saccharomyces cerevisiae | |
0.133 | - |
L-cystathionine | T85A mutant, pH 8.6, 37°C | Saccharomyces cerevisiae | |
0.418 | - |
L-cystathionine | Y158F mutant, pH 8.6, 37°C | Saccharomyces cerevisiae | |
0.45 | - |
L-serine | T81A mutant, pH 8.6, 37°C | Saccharomyces cerevisiae | |
0.56 | - |
L-cystathionine | wild-type enzyme, pH 8.6, 37°C | Saccharomyces cerevisiae | |
5.3 | - |
L-serine | S82A mutant, pH 8.6, 37°C | Saccharomyces cerevisiae | |
6.08 | - |
L-cystathionine | wild-type enzyme, pH 8.6, 37°C | Saccharomyces cerevisiae | |
8.2 | - |
L-serine | Y158F mutant, pH 8.6, 37°C | Saccharomyces cerevisiae | |
13.2 | - |
L-serine | T85A mutant, pH 8.6, 37°C | Saccharomyces cerevisiae | |
21.5 | - |
L-serine | wild-type enzyme, pH 8.6, 37°C | Saccharomyces cerevisiae |