Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.1.22 extracted from

  • Taoka, S.; Widjaja, L.; Banerjee, R.
    Assignment of enzymatic functions to specific regions of the PLP-dependent heme protein cystathionine beta-synthase (1999), Biochemistry, 38, 13155-13161.
    View publication on PubMed
Search for more literature for 4.2.1.22

Activating Compound

Activating Compound Comment Organism Structure
S-adenosyl-L-methionine activates full length enzyme, but not the truncated core, 4 S-adenosyl-L-methionine molecules per tetramer Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information CBSdeltaC143, truncated catalytic core in which the C-terminal 143 amino acid residues are deleted, higher Km for the substrates Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
 L-serine pH 6.8, 37°C, full length enzyme after L-serine pre-treatment Homo sapiens
5
-
 homocysteine pH 6.8, 37°C, full length enzyme after L-serine pre-treatment Homo sapiens
5.5
-
 L-serine pH 6.8, 37°C, full length enzyme after homocysteine pre-treatment Homo sapiens
9.7
-
 homocysteine pH 6.8, 37°C, deltaC143 mutant after L-serine pre-treatment Homo sapiens
14
-
 L-serine pH 6.8, 37°C, deltaC143 mutant after homocysteine pre-treatment Homo sapiens
15
-
 homocysteine pH 6.8, 37°C, full length enzyme after homocysteine pre-treatment Homo sapiens
18
-
 L-serine pH 6.8, 37°C, deltaC143 mutant after L-serine pre-treatment Homo sapiens
67
-
 homocysteine pH 6.8, 37°C, deltaC143 mutant after homocysteine pre-treatment Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
CBSdeltaC143 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Serine + homocysteine
-
 Homo sapiens Cystathionine + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer truncated catalytic core Homo sapiens
tetramer native enzyme Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19
-
 L-serine pH 6.8, 37°C, deltaC143 mutant after L-serine pre-treatment Homo sapiens
21
-
 L-serine pH 6.8, 37°C, full length enzyme after L-serine pre-treatment Homo sapiens
39
-
 L-serine pH 6.8, 37°C, full length enzyme after homocysteine pre-treatment Homo sapiens
45
-
 L-serine pH 6.8, 37°C, deltaC143 mutant after homocysteine pre-treatment Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme
-
 Homo sapiens
pyridoxal 5'-phosphate
-
 Homo sapiens