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Literature summary for 4.2.1.20 extracted from

  • Weyand, M.; Schlichting, I.; Marabotti, A.; Mozzarelli, A.
    Crystal structures of a new class of allosteric effectors complexed to tryptophan synthase (2002), J. Biol. Chem., 277, 10647-10652.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme complexed with allosteric effectors indole-3-acetylglycine and indole-3-acetyl-L-aspartic acid, hanging drop method, in the dark at room temperature, equal volumes of protein and reservoir solution, the latter containing 9-12% PEG 8000, 1.5 mM spermine, 1 mM EDTA, 50 mM bicine, pH 7.8, X-ray diffraction structure determination and analysis at 2.5 A resolution, modeling Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
indole-3-acetyl-L-aspartic acid inhibitor of the alpha-subunit Salmonella enterica subsp. enterica serovar Typhimurium
indole-3-acetyl-L-valine inhibitor of the alpha-subunit Salmonella enterica subsp. enterica serovar Typhimurium
indole-3-acetylglycine inhibitor of the alpha-subunit Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
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Reaction

Reaction Comment Organism Reaction ID
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(indol-3-yl)glycerol 3-phosphate alpha-subunit of the bienzyme complex, alpha-reaction Salmonella enterica subsp. enterica serovar Typhimurium D-glyceraldehyde 3-phosphate + indole
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?
L-serine + indole beta-subunit of the bienzyme complex, beta-reaction Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
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?
additional information role of allosteric effectors indole-3-acetylglycine and indole-3-acetyl-L-aspartic acid in intersubunit communication, they bind to the alpha-subunit, inhibit it and cause conformational changes that influence the beta-subunit Salmonella enterica subsp. enterica serovar Typhimurium ?
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?

Synonyms

Synonyms Comment Organism
TRPS
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Salmonella enterica subsp. enterica serovar Typhimurium