Crystallization (Comment) | Organism |
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purified enzyme complexed with allosteric effectors indole-3-acetylglycine and indole-3-acetyl-L-aspartic acid, hanging drop method, in the dark at room temperature, equal volumes of protein and reservoir solution, the latter containing 9-12% PEG 8000, 1.5 mM spermine, 1 mM EDTA, 50 mM bicine, pH 7.8, X-ray diffraction structure determination and analysis at 2.5 A resolution, modeling | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
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indole-3-acetyl-L-aspartic acid | inhibitor of the alpha-subunit | Salmonella enterica subsp. enterica serovar Typhimurium | |
indole-3-acetyl-L-valine | inhibitor of the alpha-subunit | Salmonella enterica subsp. enterica serovar Typhimurium | |
indole-3-acetylglycine | inhibitor of the alpha-subunit | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
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Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
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Reaction | Comment | Organism | Reaction ID |
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L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8) | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | D-glyceraldehyde 3-phosphate + indole | - |
? | |
L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
? | |
additional information | role of allosteric effectors indole-3-acetylglycine and indole-3-acetyl-L-aspartic acid in intersubunit communication, they bind to the alpha-subunit, inhibit it and cause conformational changes that influence the beta-subunit | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TRPS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |