Application | Comment | Organism |
---|---|---|
biotechnology | enzyme is a target for structure-based design of herbicides | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Comment) | Organism |
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purified recombinant enzyme in complex with potential alpha-reaction phosphonate inhibitors, 5-10 mg/ml protein mixed with 1 mM inhibitor, in 50 mM bicine, 1 mM Na-EDTA, 0.8-1.5 mM spermine, 12% PEG 4000, to pH 7.8 with NaOH, X-ray diffraction structure determination and analysis at 2.3 A resolution or higher | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(2-aminophenylthio)butylphosphonic acid | phosphonate inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
4-(2-hydroxy-5-fluorophenylthio)butylphosphonic acid | phosphonate inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
4-(2-hydroxyphenylsulfinyl)butylphosphonic acid | phosphonate inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
4-(2-hydroxyphenylthio)-1-butenylphosphoric acid | phosphonate inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
4-(2-hydroxyphenylthio)butylphosphonic acid | phosphonate inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
phosphonate inhibitors | mimic the transition state of the alpha-reaction with higher affinities than the natural substrate indole 3-glycerol phosphate, inhibitor binding changes the conformation of active site residues Glu49 and Phe212, stabilization of the enzyme inhibitor complex by short hydrogen bond between a phosphonate oxygen and Ser235 hydroxyl oxygen, inhibition mechanism | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(indol-3-yl)glycerol 3-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | alpha-subunit of the bienzyme complex, alpha-reaction | D-glyceraldehyde 3-phosphate + indole | - |
? | |
L-serine + indole | Salmonella enterica subsp. enterica serovar Typhimurium | beta-subunit of the bienzyme complex, beta-reaction | L-tryptophan + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O | also catalyses the conversion of serine and indole into tryptophan and water, and of indoleglycerol phosphate into indole and glyceraldehyde phosphate (the latter reaction was listed formerly as EC 4.2.1.8), detailed alpha-subunit catalytic mechanism | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(indol-3-yl)glycerol 3-phosphate | alpha-subunit of the bienzyme complex, alpha-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | D-glyceraldehyde 3-phosphate + indole | - |
? | |
L-serine + indole | beta-subunit of the bienzyme complex, beta-reaction | Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TRPS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Salmonella enterica subsp. enterica serovar Typhimurium |