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Literature summary for 4.2.1.20 extracted from

  • Ahmed, S.A.; Hyde, C.C.; Thomas, G.; Miles, E.W.
    Microcrystals of tryptophan synthase alpha2beta2 complex from Salmonella typhimurium are catalytically active (1987), Biochemistry, 26, 5492-5498.
    View publication on PubMed
Search for more literature for 4.2.1.20

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 1-(indol-3-yl)glycerol 3-phosphate reaction of alpha2beta2 complex Salmonella enterica subsp. enterica serovar Typhimurium
0.02
-
 serine serine deaminase reaction Salmonella enterica subsp. enterica serovar Typhimurium
0.04
-
 1-(indol-3-yl)glycerol 3-phosphate reaction of alpha subunit Salmonella enterica subsp. enterica serovar Typhimurium
0.06
-
 indole cosubstrate L-Ser Salmonella enterica subsp. enterica serovar Typhimurium
0.6
-
 L-Ser cosubstrate indole Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(indol-3-yl)glycerol 3-phosphate + L-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
 ?
indole + L-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?
indole-3-glycerol phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium indole + D-glyceraldehyde 3-phosphate
-
 ?
L-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 serine serine deaminase reaction Salmonella enterica subsp. enterica serovar Typhimurium
0.28
-
 1-(indol-3-yl)glycerol 3-phosphate reaction of alpha subunit Salmonella enterica subsp. enterica serovar Typhimurium
3.7
-
 1-(indol-3-yl)glycerol 3-phosphate reaction of alpha2beta2 complex Salmonella enterica subsp. enterica serovar Typhimurium
5.4
-
 indole cosubstrate L-Ser Salmonella enterica subsp. enterica serovar Typhimurium
5.4
-
 L-Ser cosubstrate indole Salmonella enterica subsp. enterica serovar Typhimurium