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Literature summary for 4.2.1.20 extracted from
- Tryptophan synthetase from Bacillus subtilis. Purification and characterization of the beta2 component (1973), J. Biol. Chem., 248, 2992-2998.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| glycerol 15% v/v stablizes beta2 subunit | Bacillus subtilis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | stimulation, Km: 140 mM | Bacillus subtilis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 82000 | - |
2 * 39700-41000, beta subunit, 1 * 82000, beta2 subunit, ultracentrifugation, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| beta2 subunit | Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, beta2-subunit, potassium phosphate buffer pH 6.5-6.6, EDTA, 2-mercaptoethanol, pyridoxal 5'-phosphate | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| indole + L-serine | catalyzed by beta2 subunit | Bacillus subtilis | L-tryptophan + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 2 * 39700-41000, beta subunit, 1 * 82000, beta2 subunit, ultracentrifugation, SDS-PAGE | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8 | beta2 subunit | Bacillus subtilis |
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