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Literature summary for 4.2.1.20 extracted from

  • Miles, E.W.; Bauerle, R.; Ahmed S.A.
    Tryptophan synthase from Escherichia coli and Salmonella thyphimurium (1987), Methods Enzymol., 142, 398-414.
    View publication on PubMed
Search for more literature for 4.2.1.20

Inhibitors

Inhibitors Comment Organism Structure
6-azido-L-tryptophan
-
 Escherichia coli
6-azido-L-tryptophan
-
 Salmonella enterica subsp. enterica serovar Typhimurium
trans-L-2-amino-4-methoxy-3-butenoic acid
-
 Escherichia coli
trans-L-2-amino-4-methoxy-3-butenoic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
alpha2beta2 holoenzyme, beta2 holoenzyme, beta2 apoenzyme, alpha subunit Salmonella enterica subsp. enterica serovar Typhimurium
alpha2beta2 holoenzyme, beta2 holoenzyme, beta2 apoenzyme, alpha subunit Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
125
-
 indole-3-glycerol phosphate + L-serine Salmonella enterica subsp. enterica serovar Typhimurium
125
-
 indole-3-glycerol phosphate + L-serine Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(indol-3-yl)glycerol 3-phosphate + L-serine catalyzed by alpha2beta2 holoenzyme Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
 ?
1-(indol-3-yl)glycerol 3-phosphate + L-serine catalyzed by alpha2beta2 holoenzyme Escherichia coli L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
 ?
2-amino-3-butenoic acid vinylglycine Salmonella enterica subsp. enterica serovar Typhimurium 2-oxobutyric acid + NH3
-
 ?
2-amino-3-butenoic acid vinylglycine Escherichia coli 2-oxobutyric acid + NH3
-
 ?
2-mercaptoethanol + L-serine + pyridoxal phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium S-pyruvylmercaptoethanol + pyridoxamine phosphate + H2O
-
 ?
2-mercaptoethanol + L-serine + pyridoxal phosphate
-
 Escherichia coli S-pyruvylmercaptoethanol + pyridoxamine phosphate + H2O
-
 ?
indole + L-serine catalyzed by beta2 subunit Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?
indole + L-serine catalyzed by beta2 subunit Escherichia coli L-tryptophan + H2O
-
 ?
indole + L-serine OH of Ser can be replaced by SCH3, OCH3 and Cl, but not by indole, indole can be replaced by CH3SH, CH2OHCH2SH, thiobenzyl alcohol, 1-propanethiol, 1-butanethiol, selenols, 6-azidoindole Salmonella enterica subsp. enterica serovar Typhimurium L-tryptophan + H2O
-
 ?
indole + L-serine OH of Ser can be replaced by SCH3, OCH3 and Cl, but not by indole, indole can be replaced by CH3SH, CH2OHCH2SH, thiobenzyl alcohol, 1-propanethiol, 1-butanethiol, selenols, 6-azidoindole Escherichia coli L-tryptophan + H2O
-
 ?
indole-3-glycerol phosphate catalyzed by alpha-subunit Salmonella enterica subsp. enterica serovar Typhimurium indole + D-glyceraldehyde 3-phosphate
-
 ?
indole-3-glycerol phosphate catalyzed by alpha-subunit Escherichia coli indole + D-glyceraldehyde 3-phosphate
-
 ?
L-serine catalyzed by beta2-subunit Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ?
L-serine catalyzed by beta2-subunit Escherichia coli pyruvate + NH3
-
 ?
L-serine OH of Ser can be replaced by SCH3, OCH3 and Cl, but not by indole Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ?
L-serine OH of Ser can be replaced by SCH3, OCH3 and Cl, but not by indole Escherichia coli pyruvate + NH3
-
 ?

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 2 mol bound per mol of beta2 subunit Salmonella enterica subsp. enterica serovar Typhimurium
pyridoxal 5'-phosphate 2 mol bound per mol of beta2 subunit Escherichia coli