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Literature summary for 4.2.1.18 extracted from

  • Wong, B.J.; Gerlt, J.A.
    Evolution of function in the crotonase superfamily: (3S)-methylglutaconyl-CoA hydratase from Pseudomonas putida (2004), Biochemistry, 43, 4646-4654.
    View publication on PubMed
Search for more literature for 4.2.1.18

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
E138A inactive mutant enzyme Pseudomonas putida
E138D the ratio of turnover number to KM-value is 9fold lower than the wild-type ratio Pseudomonas putida
E138Q the ratio of turnover number to KM-value is 3600fold lower than the wild-type ratio Pseudomonas putida
G118E no activity with crotonyl-CoA, methylcrotonyl-CoA and methylglutaconyl-CoA Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
3-methylcrotonyl-CoA
-
 Pseudomonas putida
crotonyl-CoA
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0291
-
 trans-3-methylglutaconyl-CoA pH 7.9, 25°C, wild-type enzyme Pseudomonas putida
0.043
-
 trans-3-methylglutaconyl-CoA pH 7.9, 25°C, mutant enzyme E138Q Pseudomonas putida
0.08
-
 trans-3-methylglutaconyl-CoA pH 7.9, 25°C, mutant enzyme E138D Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 PA2011
-
Pseudomonas putida PA2011
-
 PA2011
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with crotonyl-CoA and methylcrotonyl-CoA Pseudomonas putida ?
-
 ?
additional information no activity with crotonyl-CoA and methylcrotonyl-CoA Pseudomonas putida PA2011 ?
-
 ?
trans-3-methylglutaconyl-CoA + H2O
-
 Pseudomonas putida (S)-3-hydroxymethylglutaryl-CoA
-
 ?
trans-3-methylglutaconyl-CoA + H2O
-
 Pseudomonas putida PA2011 (S)-3-hydroxymethylglutaryl-CoA
-
 ?

Synonyms

Synonyms Comment Organism
(3S)-methylglutaconyl-CoA hydratase
-
 Pseudomonas putida
MGCH
-
 Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00086
-
 trans-3-methylglutaconyl-CoA pH 7.9, 25°C, mutant enzyme E138Q Pseudomonas putida
0.064
-
 trans-3-methylglutaconyl-CoA pH 7.9, 25°C, mutant enzyme E138D Pseudomonas putida
2.1
-
 trans-3-methylglutaconyl-CoA pH 7.9, 25°C, wild-type enzyme Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0317
-
 3-methylcrotonyl-CoA pH 7.09, 25°C, wild-type enzyme Pseudomonas putida
0.0949
-
 crotonyl-CoA pH 7.9, 25°C, wild-type enzyme Pseudomonas putida