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Literature summary for 4.2.1.17 extracted from

  • Nagata, R.; Kawaji, S.; Mori, Y.
    Use of enoyl coenzyme A hydratase of Mycobacterium avium subsp. paratuberculosis for the serological diagnosis of Johnes disease (2013), Vet. Immunol. Immunopathol., 155, 253-258.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene echA12 2, DNA and amino acid sequence determination and analysis, overexpression of N-terminally His-tagged enzyme in Escherichia coli in inclusion bodies Mycobacterium avium

Organism

Organism UniProt Comment Textmining
Mycobacterium avium
-
subsp. paratuberculosis, gene echA12 2 or MAP1197
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Mycobacterium avium ATCC 19698
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subsp. paratuberculosis, gene echA12 2 or MAP1197
-

Purification (Commentary)

Purification (Comment) Organism
refolded recombinant N-terminally His-tagged enzyme expresssed in Escherichia coli by nickel affinity chromatography Mycobacterium avium

Renatured (Commentary)

Renatured (Comment) Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli inclusion bodies in 20 mM Tris-HCl, 500 mM NaCl, pH 7.5, by addition of 8 M urea and protease inhibitors Mycobacterium avium