Cloned (Comment) | Organism |
---|---|
gene echA12 2, DNA and amino acid sequence determination and analysis, overexpression of N-terminally His-tagged enzyme in Escherichia coli in inclusion bodies | Mycobacterium avium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium avium | - |
subsp. paratuberculosis, gene echA12 2 or MAP1197 | - |
Mycobacterium avium ATCC 19698 | - |
subsp. paratuberculosis, gene echA12 2 or MAP1197 | - |
Purification (Comment) | Organism |
---|---|
refolded recombinant N-terminally His-tagged enzyme expresssed in Escherichia coli by nickel affinity chromatography | Mycobacterium avium |
Renatured (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged enzyme from Escherichia coli inclusion bodies in 20 mM Tris-HCl, 500 mM NaCl, pH 7.5, by addition of 8 M urea and protease inhibitors | Mycobacterium avium |