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Literature summary for 4.2.1.113 extracted from

  • Taylor Ringia, E.A.; Garrett, J.B.; Thoden, J.B.; Holden, H.M.; Rayment, I.; Gerlt, J.A.
    Evolution of enzymatic activity in the enolase superfamily: functional studies of the promiscuous o-succinylbenzoate synthase from Amycolatopsis (2004), Biochemistry, 43, 224-229.
    View publication on PubMed
Search for more literature for 4.2.1.113

Protein Variants

Protein Variants Comment Organism
K163R no o-succinylbenzoate synthase activity and no N-acylamino acid racemase activity, mutant enzyme catalyzes the stereospecific exchange of the alpha-hydrogen of N-succinyl-(S)-phenylglycine with solvent hydrogen Amycolatopsis sp.
K163S no o-succinylbenzoate synthase activity and no N-acylamino acid racemase activity, mutant enzyme catalyzes the stereospecific exchange of the alpha-hydrogen of N-succinyl-(S)-phenylglycine with solvent hydrogen Amycolatopsis sp.
K263R no o-succinylbenzoate synthase activity and no N-acylamino acid racemase activity, mutant enzyme catalyzes the stereospecific exchange of the alpha-hydrogen of N-succinyl-(R)-phenylglycine with solvent hydrogen Amycolatopsis sp.
K263S no o-succinylbenzoate synthase activity and no N-acylamino acid racemase activity, mutant enzyme catalyzes the stereospecific exchange of the alpha-hydrogen of N-succinyl-(R)-phenylglycine with solvent hydrogen Amycolatopsis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.48
-
 (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
-
 Amycolatopsis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate Amycolatopsis sp. reaction in menaquinone biosynthetic pathway 4-(2-carboxyphenyl)-4-oxobutanoic acid + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Amycolatopsis sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
-
 Amycolatopsis sp. 4-(2-carboxyphenyl)-4-oxobutanoic acid + H2O
-
 ?
(1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate reaction in menaquinone biosynthetic pathway Amycolatopsis sp. 4-(2-carboxyphenyl)-4-oxobutanoic acid + H2O
-
 ?
additional information the enzyme also shows N-acylamino acid racemase activity, with the optimal substrate being the enantiomers of N-acetyl methionine Amycolatopsis sp. ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
120
-
 (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
-
 Amycolatopsis sp.