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Literature summary for 4.2.1.11 extracted from
- Enolase from Klebsiella pneumoniae and human muscle cells. I. Purification and comparative molecular studies (2009), Adv. Clin. Exp. Med., 18, 71-78.
No PubMed abstract available
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on | Homo sapiens |  |
| Mg2+ | dependent on | Klebsiella pneumoniae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
2 * 45000, SDS-PAGE | Homo sapiens |
| 47000 | - |
2 * 47000, SDS-PAGE | Klebsiella pneumoniae |
| 94000 | - |
native enzyme, gel filtration | Klebsiella pneumoniae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Klebsiella pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE Sephadex A-50 gel filtration, Sephadex G-100 gel filtration | Klebsiella pneumoniae |
| ammonium sulfate precipitation, DEAE-Sephadex A-50 gel filtration, CM-Sephadex gel filtration, and QAE-Sephadex gel filtration | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.41 | - |
crude extract, at pH 7.8 | Klebsiella pneumoniae |
| 0.9 | - |
crude extract, at pH 6.8 | Homo sapiens |
| 31.2 | - |
after 76.1fold purification, at pH 7.8 | Klebsiella pneumoniae |
| 75 | - |
after 83fold purification, at pH 6.8 | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Homo sapiens | phosphoenolpyruvate + H2O | - |
r | |
| 2-phospho-D-glycerate | - |
Klebsiella pneumoniae | phosphoenolpyruvate + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 45000, SDS-PAGE | Homo sapiens |
| homodimer | 2 * 47000, SDS-PAGE | Klebsiella pneumoniae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2-phosphopyruvate hydrolyase | - |
Homo sapiens |
| 2-phosphopyruvate hydrolyase | - |
Klebsiella pneumoniae |
| enolase | - |
Homo sapiens |
| enolase | - |
Klebsiella pneumoniae |
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Release 2023.1 (January 2023)
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