Literature summary for 4.2.1.11 extracted from

Pietkiewicz, J.; Bednarz-Misa, I.; Jermakow, K.; Gamian, A.
Enolase from Klebsiella pneumoniae and human muscle cells II. Kinetic parameters and sensitivity to fluoride and phosphate inhibitors (2009), Adv. Clin. Exp. Med., 18, 221-233.

No PubMed abstract available

Search for more literature for 4.2.1.11

Inhibitors

Inhibitors	Comment	Organism	Structure
fluoride	the inhibitory effect of fluoride alone is noncompetitive, but it is competitive in the presence of a low phosphate level	Homo sapiens
fluoride	the inhibitory effect of fluoride alone is noncompetitive, but it is competitive in the presence of a low phosphate level	Klebsiella pneumoniae
Mg2+	inhibitory in excess	Homo sapiens
Mg2+	inhibitory in excess	Klebsiella pneumoniae
Mn2+	inhibitory in excess	Homo sapiens
Mn2+	inhibitory in excess	Klebsiella pneumoniae
phosphate	at a high phosphate concentration, noncompetitive inhibition is found and at a lower concentration competitive inhibition	Homo sapiens
phosphate	at a high phosphate concentration, noncompetitive inhibition is found and at a lower concentration competitive inhibition	Klebsiella pneumoniae
Zn2+	inhibitory in excess	Homo sapiens
Zn2+	inhibitory in excess	Klebsiella pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.199	-	2-phospho-D-glycerate	in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl	Homo sapiens
0.425	-	2-phospho-D-glycerate	in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl	Klebsiella pneumoniae
0.657	-	phosphoenolpyruvate	in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl	Klebsiella pneumoniae
0.702	-	phosphoenolpyruvate	in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Homo sapiens	5737	-
cytoplasm	-	Klebsiella pneumoniae	5737	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	maximal specific activity with respect to 2-phospho-D-glycerate is achieved in the presence of 1 mM Mg2+, in the presence of 0.1 mM MnSO4 or 0.24 mM ZnSO4 (i.e. the optimal concentrations of Mn2+ or Zn2+ ions), only 46% and 14%, respectively, of the activity determined for the Mg2+-stimulated reaction is found	Homo sapiens
Mg2+	maximal specific activity with respect to 2-phospho-D-glycerate is achieved in the presence of 1 mM Mg2+, in the presence of 0.1 mM MnSO4 or 0.24 mM ZnSO4 (i.e. the optimal concentrations of Mn2+ or Zn2+ ions), only 46% and 14%, respectively, of the activity determined for the Mg2+-stimulated reaction is found	Klebsiella pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Klebsiella pneumoniae	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
muscle	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Homo sapiens	phosphoenolpyruvate + H2O	-	r
2-phospho-D-glycerate	-	Klebsiella pneumoniae	phosphoenolpyruvate + H2O	-	r

Synonyms

Synonyms	Comment	Organism
2-phospho-D-glycerate hydrolyase	-	Homo sapiens
2-phospho-D-glycerate hydrolyase	-	Klebsiella pneumoniae
enolase	-	Homo sapiens
enolase	-	Klebsiella pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
15.47	-	phosphoenolpyruvate	in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl	Klebsiella pneumoniae
34.07	-	phosphoenolpyruvate	in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl	Homo sapiens
52.7	-	2-phospho-D-glycerate	in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl	Klebsiella pneumoniae
81.68	-	2-phospho-D-glycerate	in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	-	-	Homo sapiens
7.8	-	-	Klebsiella pneumoniae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.32	-	phosphate	in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl	Homo sapiens
0.9	-	phosphate	in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl	Klebsiella pneumoniae
1.266	-	Mn2+	in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl	Klebsiella pneumoniae
1.58	-	Zn2+	in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl	Homo sapiens
2.2	-	fluoride	at low phosphate concentrations (4-6 mM), in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl	Klebsiella pneumoniae
2.34	-	Zn2+	in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl	Klebsiella pneumoniae
3.04	-	Mn2+	in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl	Homo sapiens
3.249	-	Mg2+	in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl	Klebsiella pneumoniae
14.37	-	Mg2+	in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl	Homo sapiens
18.8	-	fluoride	at low phosphate concentrations (4-6 mM), in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
23.5	-	phosphoenolpyruvate	in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl	Klebsiella pneumoniae
48.5	-	phosphoenolpyruvate	in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl	Homo sapiens
124	-	2-phospho-D-glycerate	in 50 mM imidazole-HCl buffer, pH 7.8, with 1 mM MgSO4, 0.4 M KCl	Klebsiella pneumoniae
410	-	2-phospho-D-glycerate	in 50 mM imidazole-HCl buffer, pH 6.8, with 3 mM MgSO4, 0.4 M KCl	Homo sapiens

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Release 2023.1 (January 2023)