Application | Comment | Organism |
---|---|---|
molecular biology | studies on mitochondrial import machinery of Saccharomyces cerevisiae | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
over-expression in Escherichia coli strain BL21, expression vector pET3a, His-tagged recombinant protein | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | engineered enolase forms disrupted in catalytic activity retain features to direct mitochondrial import of tRNA | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | up to 7% of enolase activity associated with mitochondria, targeting towards the mitochondrial outer membrane, affinity to the mitochondrial surface more than 10fold higher for Eno2p than for Eno1p | Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
gel electrophoresis | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Saccharomyces cerevisiae | - |
phosphoenolpyruvate + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
W303 | - |
Purification (Comment) | Organism |
---|---|
gel filtration, for in vitro import assays into mitochondria and three-hybrid analysis | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
additional and independent function beyond glycolytic enzyme function, involvement in mitochondrial tRNA targeting, depletion of enolase inhibits tRNA import in vivo, activity of enolase as an alternative molecular chaperone suggested | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | - |
Saccharomyces cerevisiae | phosphoenolpyruvate + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
enolase | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | specific affinity to tRNA imported into mitochondria, involved to direct mitochondrial tRNA import in vitro | Saccharomyces cerevisiae |