Literature summary for 4.2.1.11 extracted from

Lee, J.H.; Kang, H.K.; Moon, Y.H.; Cho, D.L.; Kim, D.; Choe, J.Y.; Honzatko, R.; Robyt, J.F.
Cloning, expression and characterization of an extracellular enolase from Leuconostoc mesenteroides (2006), FEMS Microbiol. Lett., 259, 240-248.

Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)pLysS using the pRSETA expression vector, recombinant His-tagged protein	Leuconostoc mesenteroides

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop method, from preparations of purified dextransucrase	Leuconostoc mesenteroides

Inhibitors

Inhibitors	Comment	Organism	Structure
F-	non-competitive inhibition without phosphate and in presence of 1 mM phosphate, competitive inhibition in presence of 20 mM phosphate	Leuconostoc mesenteroides
additional information	recombinant enolase inhibits activity of purified dextransucrase	Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.61	-	2-phospho-D-glycerate	at pH 6.8	Leuconostoc mesenteroides

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47550	-	deduced from sequence	Leuconostoc mesenteroides
51400	-	SDS-PAGE	Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-phospho-D-glycerate	Leuconostoc mesenteroides	-	phosphoenolpyruvate + H2O	-	?
2-phospho-D-glycerate	Leuconostoc mesenteroides B-512FMCM	-	phosphoenolpyruvate + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Leuconostoc mesenteroides	Q1ET73	constitutively secreting dextransucrase	-
Leuconostoc mesenteroides B-512FMCM	Q1ET73	constitutively secreting dextransucrase	-

Purification (Commentary)

Purification (Comment)	Organism
extracellular enolase identified from isolates of extracellular dextransucrase from cultures of Leuconostoc mesenteroides, accumulation as small crystals	Leuconostoc mesenteroides

Source Tissue

Source Tissue	Comment	Organism	Textmining
culture medium	strain B-512FMCM	Leuconostoc mesenteroides	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activity of recombinant protein spectrophotometrically measured by conversion of 2-phospho-D-glycerate to phosphoenolpyruvate, substrate concentrations of 3 mM	Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Leuconostoc mesenteroides	phosphoenolpyruvate + H2O	-	?
2-phospho-D-glycerate	-	Leuconostoc mesenteroides B-512FMCM	phosphoenolpyruvate + H2O	-	?

Subunits

Subunits	Comment	Organism
More	extracellular enolase predicted as octamer, with two of its subunits defining the asymmetric unit of the crystal	Leuconostoc mesenteroides

Synonyms

Synonyms	Comment	Organism
enolase	-	Leuconostoc mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	activity assay at	Leuconostoc mesenteroides

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	-	optimum determined by activity assay	Leuconostoc mesenteroides

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8.3	catalytic activity above and below pH 6.8 remains constant at 30% of maximal activity	Leuconostoc mesenteroides

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
4.6	-	F-	in presence of 1 mM phosphate	Leuconostoc mesenteroides
5.4	-	F-	in absence of phosphate	Leuconostoc mesenteroides
9.2	-	F-	in presence of 20 mM phosphate	Leuconostoc mesenteroides

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)