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Literature summary for 4.2.1.11 extracted from

  • Keller, A.; Peltzer, J.; Carpentier, G.; Horvath, I.; Olah, J.; Duchesnay, A.; Orosz, F.; Ovadi, J.
    Interactions of enolase isoforms with tubulin and microtubules during myogenesis (2007), Biochim. Biophys. Acta, 1770, 919-926.
    View publication on PubMed
Search for more literature for 4.2.1.11

Inhibitors

Inhibitors Comment Organism Structure
2-phosphoglycerate presence of 0.8 mM 2-phosphoglycerate abolished the binding of beta,beta-enolase to tubulin, kinetics shown Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic fractions of myoblasts and myotubes, microtubule-association of alpha,alpha-enolase in undifferentiated myoblasts and in myotubes, also nuclear localization in myoblasts but not in myotubes determined, role of enolases in cytoskeletal dynamics during myoblast differentiation assumed Mus musculus 5829
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cytosol nuclear localization in myoblasts not detected, direct interaction with microtubules and tubulin during myoblast differentiation identified, role in cytoskeletal dynamics during myoblast differentiation assumed Mus musculus 5829
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Mus musculus
-
 phosphoenolpyruvate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus P17182 alpha,alpha-enolase
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Mus musculus P21550 beta,beta-enolase
-

Purification (Commentary)

Purification (Comment) Organism
for localization studies, immunochemistry and confocal microscopy Mus musculus
gel filtration, for localization studies and tubulin-binding kinetics Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle alpha,beta heterodimer and beta,beta homodimer in striated muscle, interaction with tubulin during differentiation of muscle satellite cells but not in undifferentiated myoblasts, immobilized beta,beta-enolase interacts with tubulin in vitro Mus musculus
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skeletal muscle satellite cell differentiation, alpha,alpha-enolase highly expressed during early ontogenesis, concentration of alpha,alpha-enolase in skeletal muscle varies from 0.1 mM to 0.01 mM until differentiation, binding affinity to tubulin is about 0.2 microM, alpha,beta heteroassociation with tubulin in muscle under physiological conditions Mus musculus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme activity measured with the natural substrate 2-phospho-D-glycerate, conversion of reaction products determined by spectroscopy, kinetics of binding studies to tubulin by ELISA and surface plasmon resonance Mus musculus
additional information
-
 enzyme activity tested, kinetics of binding studies to tubulin estimated by ELISA and surface plasmon resonance, association of beta,beta enolase to microtubules in differentiating myotubes but not in myoblasts Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Mus musculus phosphoenolpyruvate + H2O
-
 ?
2-phospho-D-glycerate interaction studies between alpha,alpha enolase and tubulin, co-localization studies with microtubules Mus musculus phosphoenolpyruvate + H2O
-
 ?

Synonyms

Synonyms Comment Organism
enolase
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 enzyme activity assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 enzyme activity assay at Mus musculus