Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain XL1Blue as an N-terminal His6-tagged protein | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | inhibitory at higher concentrations | Plasmodium falciparum | |
Na+ | 50% inhibition around 0.3-0.4 M | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
(25R)-3beta-hydroxycholest-5-en-27-oate | 2-phospho-D-glycerate | Plasmodium falciparum | |
0.25 | - |
(25R)-3beta-hydroxycholest-5-en-27-oate | phosphoenolpyruvate | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | slightly activating effect | Plasmodium falciparum | |
Mg2+ | - |
Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25710 | - |
MALDI-TOF, double charged monomer | Plasmodium falciparum |
50000 | - |
SDS-PAGE | Plasmodium falciparum |
51380 | - |
MALDI-TOF, single charged monomer | Plasmodium falciparum |
51400 | - |
MALDI-TOF | Plasmodium falciparum |
100000 | - |
gel filtration | Plasmodium falciparum |
102800 | - |
MALDI-TOF, double charged dimer | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phospho-D-glycerate | Plasmodium falciparum | - |
phosphoenolpyruvate + H2O | - |
r | |
2-phospho-D-glycerate | Plasmodium falciparum NF54 | - |
phosphoenolpyruvate + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Plasmodium falciparum NF54 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-nitrilotriacetic acid affinity chromatography, eluted with imidazole | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30 | - |
2-phospho-D-glycerate, pH 7.4, 20°C, Tris/HCl | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(25R)-3beta-hydroxycholest-5-en-27-oate | - |
Plasmodium falciparum | ? | - |
? | |
(25R)-3beta-hydroxycholest-5-en-27-oate | - |
Plasmodium falciparum NF54 | ? | - |
? | |
2-phospho-D-glycerate | - |
Plasmodium falciparum | phosphoenolpyruvate + H2O | - |
r | |
2-phospho-D-glycerate | - |
Plasmodium falciparum NF54 | phosphoenolpyruvate + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000, gel filtration | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
r-Pfen | - |
Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.6 | irrespective of substrate | Plasmodium falciparum |