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Literature summary for 4.2.1.11 extracted from

  • Pal-Bhowmick, I.; Sadagopan, K.; Vora, H.K.; Sehgal, A.; Sharma, S.; Jarori, G.K.
    Cloning, over-expression, purification and characterization of Plasmodium falciparum enolase (2004), Eur. J. Biochem., 271, 4845-4854.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain XL1Blue as an N-terminal His6-tagged protein Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ inhibitory at higher concentrations Plasmodium falciparum
Na+ 50% inhibition around 0.3-0.4 M Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
(25R)-3beta-hydroxycholest-5-en-27-oate 2-phospho-D-glycerate Plasmodium falciparum
0.25
-
(25R)-3beta-hydroxycholest-5-en-27-oate phosphoenolpyruvate Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
K+ slightly activating effect Plasmodium falciparum
Mg2+
-
Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25710
-
MALDI-TOF, double charged monomer Plasmodium falciparum
50000
-
SDS-PAGE Plasmodium falciparum
51380
-
MALDI-TOF, single charged monomer Plasmodium falciparum
51400
-
MALDI-TOF Plasmodium falciparum
100000
-
gel filtration Plasmodium falciparum
102800
-
MALDI-TOF, double charged dimer Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-phospho-D-glycerate Plasmodium falciparum
-
phosphoenolpyruvate + H2O
-
r
2-phospho-D-glycerate Plasmodium falciparum NF54
-
phosphoenolpyruvate + H2O
-
r

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-
Plasmodium falciparum NF54
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-nitrilotriacetic acid affinity chromatography, eluted with imidazole Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
30
-
2-phospho-D-glycerate, pH 7.4, 20°C, Tris/HCl Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(25R)-3beta-hydroxycholest-5-en-27-oate
-
Plasmodium falciparum ?
-
?
(25R)-3beta-hydroxycholest-5-en-27-oate
-
Plasmodium falciparum NF54 ?
-
?
2-phospho-D-glycerate
-
Plasmodium falciparum phosphoenolpyruvate + H2O
-
r
2-phospho-D-glycerate
-
Plasmodium falciparum NF54 phosphoenolpyruvate + H2O
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 50000, gel filtration Plasmodium falciparum

Synonyms

Synonyms Comment Organism
r-Pfen
-
Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.6 irrespective of substrate Plasmodium falciparum