Literature summary for 4.2.1.11 extracted from

Hannaert, V.; Albert, M.A.; Rigden, D.J.; da Silva Giotto, M.T.; Thiemann, O.; Garratt, R.C.; Van Roy, J.; Opperdoes, F.R.; Michels, P.A.
Kinetic characterization, structure modelling studies and crystallization of Trypanosoma brucei enolase (2003), Eur. J. Biochem., 270, 3205-3213.

Search for more literature for 4.2.1.11

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli of a his-tagged recombinant enzyme	Trypanosoma brucei

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop method	Trypanosoma brucei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.054	-	2-phospho-D-glycerate	pH 7.6, 25ºC	Trypanosoma brucei
0.244	-	phosphoenolpyruvate	pH 7.6, 25ºC	Trypanosoma brucei

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Trypanosoma brucei	5829	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	essential for activity, high concentrations are inhibitory	Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
46461	-	x * 46461, calculated from amino-acid sequence	Trypanosoma brucei

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma brucei	Q9NDH8	-	-

Purification (Commentary)

Purification (Comment)	Organism
using affinity chromatography to isolate a his-tagged recombinant enzyme followed by cleavage of the tag	Trypanosoma brucei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
85	-	pH 7.6, 25ºC	Trypanosoma brucei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phospho-D-glycerate	-	Trypanosoma brucei	phosphoenolpyruvate	-	r

Subunits

Subunits	Comment	Organism
?	x * 46461, calculated from amino-acid sequence	Trypanosoma brucei

Synonyms

Synonyms	Comment	Organism
2-phospho-D-glycerate hydrolase	-	Trypanosoma brucei
enolase	-	Trypanosoma brucei

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.7	-	25ºC	Trypanosoma brucei

pH Range

pH Minimum	pH Maximum	Comment	Organism
7.3	8	activity decreases steadily, 60% of the activity remains at pH 7.0 and pH 8.4	Trypanosoma brucei

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)