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Literature summary for 4.2.1.11 extracted from

  • Green, G.A.; Girardot, R.; Baldacini, O.; Ledig, M.; Monteil, H.
    Characterization of enolase from Clostridium difficile (1993), Curr. Microbiol., 26, 53-56.
No PubMed abstract available
Search for more literature for 4.2.1.11

Inhibitors

Inhibitors Comment Organism Structure
F- in presence of phosphate, competitive Clostridioides difficile

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration: 1 mM Clostridioides difficile
Zn2+ optimal concentration: 1 mM Clostridioides difficile

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Clostridioides difficile
300000
-
 non-denaturing PAGE Clostridioides difficile
338000
-
 gel filtration Clostridioides difficile

Organism

Organism UniProt Comment Textmining
Clostridioides difficile
-
-
-
Paeniclostridium sordellii
-
-
-
Paraclostridium bifermentans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridioides difficile
partial Paeniclostridium sordellii
partial Paraclostridium bifermentans

Storage Stability

Storage Stability Organism
-20°C, 10 mM Tris-HCL, pH 7.5, stable Clostridioides difficile

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Clostridioides difficile phosphoenolpyruvate
-
 ?
2-phospho-D-glycerate
-
 Paeniclostridium sordellii phosphoenolpyruvate
-
 ?
2-phospho-D-glycerate
-
 Paraclostridium bifermentans phosphoenolpyruvate
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Clostridioides difficile

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 50
-
 Clostridioides difficile

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 80 80°C: 27% of maximal activity Clostridioides difficile
25 80 25-50°C: maximal activity, 60°C, 89% of maximal activity, 70°C: 57% of maximal activity Clostridioides difficile

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Clostridioides difficile