Any feedback?
Literature summary for 4.2.1.11 extracted from
- Enolase isozymes from Ricinus communis: partial purification and characterization of the isozymes (1984), Arch. Biochem. Biophys., 233, 643-651.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-phosphoglycerate | competitive | Ricinus communis |  |
| F- | - |
Ricinus communis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
2-phospho-D-glycerate | plastidic isoenzyme | Ricinus communis | |
| 0.061 | - |
2-phospho-D-glycerate | cytosolic isoenzyme | Ricinus communis | |
| 2.6 | - |
phosphoenolpyruvate | plastidic isoenzyme | Ricinus communis | |
| 7.2 | - |
phosphoenolpyruvate | cytosolic isoenzyme | Ricinus communis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Ricinus communis | 5829 | - |
| plastid | - |
Ricinus communis | 9536 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | less than 40% of the activation with Mg2+ | Ricinus communis | |
| Mg2+ | absolute requirement for divalent metal ions, Mg2+ is most effective | Ricinus communis | |
| Mn2+ | Km for the cytoplasmic isoenzyme: 1.2 mM | Ricinus communis | |
| Mn2+ | absolute requirement for divalent metal ions | Ricinus communis | |
| Mn2+ | Km for the plastidic isoenzyme: 1.4 mM | Ricinus communis | |
| Mn2+ | 10% of the activation with Mg2+ | Ricinus communis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ricinus communis | - |
L. Cv. Baker 296 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Ricinus communis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | developing | Ricinus communis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 197.4 | - |
plastidic isoenzyme | Ricinus communis |
| 200 | - |
- |
Ricinus communis |
| 252.4 | - |
cytosolic isoenzyme | Ricinus communis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phospho-D-glycerate | - |
Ricinus communis | phosphoenolpyruvate | - |
r | |
| 2-phospho-D-glycerate | - |
Ricinus communis | phosphoenolpyruvate + H2O | - |
? | |
| phosphoenolpyruvate | - |
Ricinus communis | 2-phospho-D-glycerate | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
formation of 2-phosphoglycerate, plastidic isoenzyme | Ricinus communis |
| 7.5 | - |
formation of phosphoenolpyruvate | Ricinus communis |
| 7.8 | - |
formation of 2-phosphoglycerate, cytosolic isoenzyme | Ricinus communis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
