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Literature summary for 4.2.1.11 extracted from

  • Oh, S.K.; Brewer, J.M.
    Purification and properties of enolase from swine kidney (1973), Arch. Biochem. Biophys., 157, 491-499.
    View publication on PubMed
Search for more literature for 4.2.1.11

General Stability

General Stability Organism
Mg2+, 20% sucrose or glycerol stabilize enzyme in dilute solutions Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
2-phosphoglycerate substrate inhibition Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Sus scrofa
0.05
-
 2-phospho-D-glycerate
-
 Sus scrofa
0.11
-
 phosphoenolpyruvate
-
 Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
KCl required for maximal activity Sus scrofa
Mg2+ Km: 0.43 mM Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 2 * 52000, SDS-PAGE Sus scrofa
88000
-
 sedimentation velocity measurement Sus scrofa
110000
-
 gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-

Storage Stability

Storage Stability Organism
4°C, 1 mg/ml or more, 0.1 M imidazole HCl, pH 7.0 and 2 mM MgCl2, stable for at least 2 months Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
 Sus scrofa phosphoenolpyruvate
-
 r
2-phospho-D-glycerate
-
 Sus scrofa phosphoenolpyruvate + H2O
-
 ?
phosphoenolpyruvate
-
 Sus scrofa 2-phospho-D-glycerate
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 52000, SDS-PAGE Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Sus scrofa