Literature summary for 4.1.99.3 extracted from

Albarracin, V.H.; Simon, J.; Pathak, G.P.; Valle, L.; Douki, T.; Cadet, J.; Borsarelli, C.D.; Farias, M.E.; Gaertner, W.
First characterisation of a CPD-class I photolyase from a UV-resistant extremophile isolated from high-altitude Andean lakes (2014), Photochem. Photobiol. Sci., 13, 739-750.

Search for more literature for 4.1.99.3

Cloned(Commentary)

Cloned (Comment)	Organism
gene CL42_09140, DNA and amino acid equence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3), expression of the enzyme Ver3Phr in and functional complementation of an Escherichia coli strain KY1225 deficient in photolyase	Acinetobacter sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	about, recombinant His-tagged enzyme, gel filtration and mass spectrometry	Acinetobacter sp.

Organism

Organism	UniProt	Comment	Textmining
Acinetobacter sp.	F5ASF3	gene CL42_09140; UV-resistant strain isolated from high-altitude Andean lakes in Argentinean Puna, gene CL42_09140	-
Acinetobacter sp. Ver3	F5ASF3	gene CL42_09140; UV-resistant strain isolated from high-altitude Andean lakes in Argentinean Puna, gene CL42_09140	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and gel filtration	Acinetobacter sp.

Subunits

Subunits	Comment	Organism
monomer	1 * 54700-60000, recombinant His-tagged enzyme, SDS-PAGE and sequence calculation	Acinetobacter sp.

Synonyms

Synonyms	Comment	Organism
CPD-class I photolyase	-	Acinetobacter sp.
cyclobutane pyrimidine dimer-class I photolyase	-	Acinetobacter sp.
Ver3Phr	-	Acinetobacter sp.

Cofactor

Cofactor	Comment	Organism	Structure
5,10-methenyltetrahydrofolate	FAD and 5,10-methenyltetrahydrofolate act as chromophore and antenna molecules, respectively	Acinetobacter sp.
FAD	FAD and 5,10-methenyltetrahydrofolate act as chromophore and antenna molecules, respectively. The Ver3 chromophore always remains partly (including the semiquinone state) or fully reduced under all experimental conditions tested	Acinetobacter sp.

General Information

General Information	Comment	Organism
evolution	phylogenetic analysis and tree, overview	Acinetobacter sp.

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Release 2023.1 (January 2023)