Literature summary for 4.1.99.3 extracted from

Azizoglu, S.; Kizilel, R.; Marusic, M.; Kavakli, I.H.; Erman, B.; Kizilel, S.
Computational and experimental investigation of DNA repair protein photolyase interactions with low molecular weight drugs (2013), J. Mol. Recognit., 26, 297-307.

Search for more literature for 4.1.99.3

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of the enzyme from VcPhr genes in pmal-c2x bacterial expression vector in Escherichia coli strain UNC 523	Vibrio cholerae serotype O1

Protein Variants

Protein Variants	Comment	Organism
additional information	immobilization of recombinant purified DNA photolyase using avidin-biotin-based immobilization method with neutravidin-modified BioCap chip as a sensor surface	Vibrio cholerae serotype O1

Inhibitors

Inhibitors	Comment	Organism	Structure
azelastine	-	Vibrio cholerae serotype O1
clomifene	-	Vibrio cholerae serotype O1
desloratidine	-	Vibrio cholerae serotype O1
fenofibrate	-	Vibrio cholerae serotype O1
lornoxicam	-	Vibrio cholerae serotype O1
additional information	DNA repair protein photolyase as a model protein, because it is the most widely studied enzyme among its family members including photolyases and cryptochromes with a well-known three-dimensional structure, for characterization of interactions of the FAD region of photolyase with available drugs, which significant to determine the possible unknown effects of known drugs on the suppressor activity of human cryptosome on CLOCK. Cryptochromes act as negative transcriptional regulator of CLOCK in mammals. Molecular docking of drugs to the FAD region of Vibrio cholerae DNA photolyase, overview	Vibrio cholerae serotype O1
Prednisone	-	Vibrio cholerae serotype O1
rupatadine	-	Vibrio cholerae serotype O1
Triamcinolone	-	Vibrio cholerae serotype O1

Organism

Organism	UniProt	Comment	Textmining
Vibrio cholerae serotype O1	-	-	-

Synonyms

Synonyms	Comment	Organism
DNA photolyase	-	Vibrio cholerae serotype O1
DNA repair protein photolyase	-	Vibrio cholerae serotype O1
photolyase	-	Vibrio cholerae serotype O1
VcPHR	-	Vibrio cholerae serotype O1

Cofactor

Cofactor	Comment	Organism	Structure
FAD	the FAD binding region is required for the catalytic activity of DNA photolyase	Vibrio cholerae serotype O1

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
additional information	-	additional information	drug ligand binding follows pseudo-first-order kinetics, equivalent and independent for all binding sites, theoretical docking and dissociation constants, overview	Vibrio cholerae serotype O1

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)