Literature summary for 4.1.99.3 extracted from

Thiagarajan, V.; Byrdin, M.; Eker, A.P.; Mueller, P.; Brettel, K.
Kinetics of cyclobutane thymine dimer splitting by DNA photolyase directly monitored in the UV (2011), Proc. Natl. Acad. Sci. USA, 108, 9402-9407.

Search for more literature for 4.1.99.3

Cloned(Commentary)

Cloned (Comment)	Organism
-	Aspergillus nidulans

Organism

Organism	UniProt	Comment	Textmining
Aspergillus nidulans	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA)	using a transient absorption setup, cyclobutane thymine dimer repair in the main UV absorption band of intact thymine at 266 nm is monitored. Flavin transitions that overlay DNA-based absorption changes at 266 nm are monitored independently in the visible and subtracted to obtain the true repair kinetics. Restoration of intact thymine show a short lag and a biexponential rise with time constants of 0.2 and 1.5 ns. The two time constants are assigned to splitting of the intradimer bonds (creating one intact thymine and one thymine anion radical T-) and electron return from T- to the FAD cofactor with recovery of the second thymine, respectively	Aspergillus nidulans	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cyclobutadipyrimidine in DNA	the substrate is a modified thymidine 10-mer with a central T = T and all other bases, except the one at the 3' end, replaced by 5,6-dihydrothymine (5S:5R stereoisomer ratio 90:10)	Aspergillus nidulans	pyrimidine residues in DNA	-	?

Synonyms

Synonyms	Comment	Organism
CPD photolyase	-	Aspergillus nidulans

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Aspergillus nidulans

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Release 2023.1 (January 2023)