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Literature summary for 4.1.99.3 extracted from

  • Chaves, I.; Pokorny, R.; Byrdin, M.; Hoang, N.; Ritz, T.; Brettel, K.; Essen, L.O.; van der Horst, G.T.; Batschauer, A.; Ahmad, M.
    The cryptochromes: blue light photoreceptors in plants and animals (2011), Annu. Plant Biol., 62, 335-364.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Synechococcus elongatus PCC 7942 = FACHB-805
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Reaction

Reaction Comment Organism Reaction ID
cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) on top of the transient formation of tryptophan radicals during photoactivation, evidence is found for oxidation of a tyrosine residue by a tryptophan radical. The tyrosine radical thus formed is reduced by an extrinsic reductant, suggesting that in this case the terminal intrinsic electron donor is tyrosine rather than tryptophan Synechococcus elongatus PCC 7942 = FACHB-805
cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) upon absorption of a visible photon, the photoexcited flavin radical FADH abstracts an electron from nearby W382. The tryptophanyl radical W382 thus formed abstracts an electron from the nearby middle tryptophan, W359. W359 radical abstracts an electron from W306. As the second and third steps are faster than the first one, the intermediate states are not populated in wild-type photolyase to any significant extent and escape spectroscopic detection. The described forward electron transfer steps between the tryptophans are in competition with back electron transfer from FADH- to the respective tryptophanyl cation radical, so that the quantum yield of formation of the terminal W306 radical is only 20%. W306 radical releases a proton to the aqueous phase in 200 ns. The resulting neutral radical W306 radical can be reduced by extrinsic reductants, leaving a photolyase that contains FADH as required for photorepair of DNA Escherichia coli

Synonyms

Synonyms Comment Organism
CPD photolyase
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Synechococcus elongatus PCC 7942 = FACHB-805
CPD photolyase
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Escherichia coli

Cofactor

Cofactor Comment Organism Structure
deazaflavin antenna cofactor Synechococcus elongatus PCC 7942 = FACHB-805
FAD
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Synechococcus elongatus PCC 7942 = FACHB-805
FAD
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Escherichia coli