Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Codon Plus BL21[pREP4] cells | Arabidopsis thaliana |
GST-tagged class II CPD photolyase overexpressed in Escherichia coli | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
E109A | is catalytically active but unable to bind the second cofactor methenyltetrahydrofolate | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Escherichia coli | - |
- |
- |
Oryza sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
- |
Oryza sativa |
Storage Stability | Organism |
---|---|
-70°C, 0.05 M sodium phosphate, pH 7.0, 0.1 M NaCl, 20% (v/v) glycerol | Escherichia coli |
-70°C, 0.05 M sodium phosphate, pH 7.0, 0.1 M NaCl, 20% (v/v) glycerol | Arabidopsis thaliana |
-70°C, 0.05 M sodium phosphate, pH 7.0, 0.1 M NaCl, 20% (v/v) glycerol | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
class I CPD photolyase | - |
Escherichia coli |
class I cyclobutane pyrimidine dimer photolyase | - |
Escherichia coli |
class I photolyase | - |
Escherichia coli |
class II CPD photolyase | - |
Arabidopsis thaliana |
class II CPD photolyase | - |
Oryza sativa |
class II cyclobutane pyrimidine dimer photolyase | - |
Arabidopsis thaliana |
class II cyclobutane pyrimidine dimer photolyase | - |
Oryza sativa |
class II photolyase | - |
Arabidopsis thaliana |
class II photolyase | - |
Oryza sativa |
CPD photolyase | - |
Escherichia coli |
CPD photolyase | - |
Arabidopsis thaliana |
CPD photolyase | - |
Oryza sativa |
CPDI | - |
Escherichia coli |
CPDII | - |
Arabidopsis thaliana |
CPDII | - |
Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli | |
FAD | binds the flavin cofactor in a pocket that is conserved in terms of its electronic properties | Arabidopsis thaliana | |
FAD | binds the flavin cofactor in a pocket that is conserved in terms of its electronic properties. W399-W378-W406 may function as potential electron donors to the flavin and are possible candidate tryptophans for light-induced electron transfer | Oryza sativa | |
FADH2 | photoreduction of FADH proceeds along the conserved tryptophan triad W306-W359-W382 | Escherichia coli | |
methenyltetrahydrofolate | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | photoreduction kinetics of class II photolyases are very similar to those of the other classes with even higher photoreduction rates in class II as compared to class I. W399-W378-W406 electron-transfer pathway is conserved among class II CPD photolyase enzymes | Arabidopsis thaliana |
metabolism | photoreduction kinetics of class II photolyases are very similar to those of the other classes with even higher photoreduction rates in class II as compared to class I. W399-W378-W406 electron-transfer pathway is conserved among class II CPD photolyase enzymes | Oryza sativa |