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Literature summary for 4.1.99.3 extracted from

  • Okafuji, A.; Biskup, T.; Hitomi, K.; Getzoff, E.D.; Kaiser, G.; Batschauer, A.; Bacher, A.; Hidema, J.; Teranishi, M.; Yamamoto, K.; Schleicher, E.; Weber, S.
    Light-induced activation of class II cyclobutane pyrimidine dimer photolyases (2010), DNA Repair, 9, 495-505.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Codon Plus BL21[pREP4] cells Arabidopsis thaliana
GST-tagged class II CPD photolyase overexpressed in Escherichia coli Oryza sativa

Protein Variants

Protein Variants Comment Organism
E109A is catalytically active but unable to bind the second cofactor methenyltetrahydrofolate Escherichia coli

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-
Escherichia coli
-
-
-
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Arabidopsis thaliana
-
Oryza sativa

Storage Stability

Storage Stability Organism
-70°C, 0.05 M sodium phosphate, pH 7.0, 0.1 M NaCl, 20% (v/v) glycerol Escherichia coli
-70°C, 0.05 M sodium phosphate, pH 7.0, 0.1 M NaCl, 20% (v/v) glycerol Arabidopsis thaliana
-70°C, 0.05 M sodium phosphate, pH 7.0, 0.1 M NaCl, 20% (v/v) glycerol Oryza sativa

Synonyms

Synonyms Comment Organism
class I CPD photolyase
-
Escherichia coli
class I cyclobutane pyrimidine dimer photolyase
-
Escherichia coli
class I photolyase
-
Escherichia coli
class II CPD photolyase
-
Arabidopsis thaliana
class II CPD photolyase
-
Oryza sativa
class II cyclobutane pyrimidine dimer photolyase
-
Arabidopsis thaliana
class II cyclobutane pyrimidine dimer photolyase
-
Oryza sativa
class II photolyase
-
Arabidopsis thaliana
class II photolyase
-
Oryza sativa
CPD photolyase
-
Escherichia coli
CPD photolyase
-
Arabidopsis thaliana
CPD photolyase
-
Oryza sativa
CPDI
-
Escherichia coli
CPDII
-
Arabidopsis thaliana
CPDII
-
Oryza sativa

Cofactor

Cofactor Comment Organism Structure
FAD
-
Escherichia coli
FAD binds the flavin cofactor in a pocket that is conserved in terms of its electronic properties Arabidopsis thaliana
FAD binds the flavin cofactor in a pocket that is conserved in terms of its electronic properties. W399-W378-W406 may function as potential electron donors to the flavin and are possible candidate tryptophans for light-induced electron transfer Oryza sativa
FADH2 photoreduction of FADH proceeds along the conserved tryptophan triad W306-W359-W382 Escherichia coli
methenyltetrahydrofolate
-
Escherichia coli

General Information

General Information Comment Organism
metabolism photoreduction kinetics of class II photolyases are very similar to those of the other classes with even higher photoreduction rates in class II as compared to class I. W399-W378-W406 electron-transfer pathway is conserved among class II CPD photolyase enzymes Arabidopsis thaliana
metabolism photoreduction kinetics of class II photolyases are very similar to those of the other classes with even higher photoreduction rates in class II as compared to class I. W399-W378-W406 electron-transfer pathway is conserved among class II CPD photolyase enzymes Oryza sativa