Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
N378S | the recombinant mutant photolyase contains oxidized FAD (FADox) but not FADH after routine purification procedures, the mutant protein contains FADH in vivo as the wild type enzyme, the mutant photolyase is photoreducible and capable of binding cyclobutadipyrimidine dimers in DNA, but catalytically inert | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ chelating Sepharose column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclobutadipyrimidine in DNA | catalyzes the repair of cyclobutadipyrimidine dimers in DNA under near-UV or blue light irradiation | Escherichia coli | pyrimidine residues in DNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA photolyase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | the catalytic activity of the enzyme requires fully reduced FAD | Escherichia coli |