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Literature summary for 4.1.99.3 extracted from

  • Takeuchi, Y.; Inoue, T.; Takemura, K.; Hada, M.; Takahashi, S.; Ioki, M.; Nakajima, N.; Kondo, N.
    Induction and inhibition of cyclobutane pyrimidine dimer photolyase in etiolated cucumber (Cucumis sativus) cotyledons after ultraviolet irradiation depends on wavelength (2007), J. Plant Res., 120, 365-374.
    View publication on PubMed
Search for more literature for 4.1.99.3

Inhibitors

Inhibitors Comment Organism Structure
H2O2 addition of H2O2 to the enzyme solution also inactivates CPD photolyase activity Cucumis sativus

Organism

Organism UniProt Comment Textmining
Cucumis sativus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protein is purified by use of a Sephadex G-25 spin column and a DEAE-Sephacel column Cucumis sativus

Source Tissue

Source Tissue Comment Organism Textmining
additional information level of CsPHR transcripts increase immediately after the start of UV-irradiation and gradually decrease after 4 h. After irradiation for 10 h the transcript level is almost the same as the initial level Cucumis sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 monochromatic UV irradiation at wavelengths above 300 nm increases the level of CsPHR transcripts similarly to irradiation at wavelengths below 320 nm, but reduces CPD photolyase activity compared with the dark control. Exposure of a CPD photolyase solution to UV-C (254 nm) reduces enzyme activity and induces accumulation of H2O2. Addition of H2O2 to the enzyme solution also inactivates CPD photolyase activity Cucumis sativus
additional information
-
 polychromatic UV- irradiation increases activity of CPD photolyase by an amount which depends on UV irradiance, and the level of CsPHR transcripts temporarily increases before the activity reaches a constant level. UV light above 320 nm is more effective than visible light at increasing CPD photolyase activity Cucumis sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclobutadipyrimidine in DNA
-
 Cucumis sativus 2 pyrimidine residues in DNA
-
 ?

Synonyms

Synonyms Comment Organism
CPD photolyase
-
 Cucumis sativus
CsPHR
-
 Cucumis sativus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Cucumis sativus