Cloned (Comment) | Organism |
---|---|
cloned in Escherichia coli as a C-terminal His-tagged fusion protein | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by using Ni2+ chelating Sepharose Fast Flow columns and Sephadex gel filtration resins. Methenyltetrahydrofolate-free photolyase is obtained by binding the C-terminal His-tagged holoenzyme to a metal-affinity column at neutral pH and washing the column with deionized water. The defolated enzyme can be eluated with 0.5 M imidazole pH 7.2. Apo-photolyase is obtained by treating the His-tagged holoenzyme with 0.5 M imidazole pH 10.0 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of freshly prepared holoenzyme methenyltetrahydrofolate + FADH is considered 100%. After methenyltetrahydrofolate cofactor removal the activity of the enzyme decreases to 70%, reconstituted oxidized photolyase shows the lowest activity with 34%. Activity after dithionite reduction: 83% | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
DNA photolyase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli | |
methenyltetrahydrofolate | - |
Escherichia coli |