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Literature summary for 4.1.99.3 extracted from

  • Hitomi, K.; Nakamura, H.; Kim, S.T.; Mizukoshi, T.; Ishikawa, T.; Iwai, S.; Todo, T.
    Role of two histidines in the (6-4) photolyase reaction (2001), J. Biol. Chem., 276, 10103-10109.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
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Xenopus laevis

Protein Variants

Protein Variants Comment Organism
H354A affinity to substrate is comparable to wild-type enzyme, no activity Xenopus laevis
H358A affinity to substrate is comparable to wild-type enzyme, no activity Xenopus laevis
L355A low affinity to the substrate Xenopus laevis
Q288A affinity to substrate is comparable to wild-type enzyme Xenopus laevis
W291A affinity to substrate is comparable to wild-type enzyme, mutant retains some activity Xenopus laevis
W398A affinity to substrate is comparable to wild-type enzyme, mutant retains some activity Xenopus laevis

Organism

Organism UniProt Comment Textmining
Xenopus laevis
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-
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Reaction

Reaction Comment Organism Reaction ID
cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) mechanism Xenopus laevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclobutadipyrimidine in DNA
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Xenopus laevis 2 pyrimidine residues in DNA
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?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
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-
Xenopus laevis

pH Range

pH Minimum pH Maximum Comment Organism
7 10 about 50% activity at pH 7, about 75% activity at pH 10 Xenopus laevis