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Literature summary for 4.1.99.2 extracted from

  • Seisser, B.; Zinkl, R.; Gruber, K.; Kaufmann, F.; Hafner, A.; Kroutil, W.
    Cutting long syntheses short: Access to non-natural tyrosine derivatives employing an engineered tyrosine phenol lyase (2010), Adv. Synth. Catal., 352, 731-736.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Citrobacter freundii

Protein Variants

Protein Variants Comment Organism
F36V changed substrate specificity Citrobacter freundii
F448V changed substrate specificity Citrobacter freundii
M288V changed substrate specificity Citrobacter freundii
M379V changed substrate specificity Citrobacter freundii
M379V site-directed mutagenesis, the mutant is active with o-cresol, o-methoxyphenol, and o-chlorophenol, as well as 3-methyl-, 3-methoxy-, 3 -fluoro, and 3-chloro-L-tyrosine in contrast to the wild-type enzyme Citrobacter freundii
T125S changed substrate specificity Citrobacter freundii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + H2O Citrobacter freundii
-
phenol + pyruvate + NH3
-
r

Organism

Organism UniProt Comment Textmining
Citrobacter freundii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-chloro-L-tyrosine + H2O substrate of mutant M379V, not of the wild-type enzyme Citrobacter freundii ?
-
?
3-fluoro-L-tyrosine + H2O substrate of mutant M379V, not of the wild-type enzyme Citrobacter freundii ?
-
?
3-methoxy-L-tyrosine + H2O substrate of mutant M379V, not of the wild-type enzyme Citrobacter freundii ?
-
?
3-methyl-L-tyrosine + H2O substrate of mutant M379V, not of the wild-type enzyme Citrobacter freundii ?
-
?
L-tyrosine + H2O
-
Citrobacter freundii phenol + pyruvate + NH3
-
r
additional information substrate specificity with 3-substituted tyrosines, the wild-type enzyme shows a narrow substrate spectrum, overview. Mutant M379V converts several L-tyrosine derivatives to the correspondent products Citrobacter freundii ?
-
?
o-chlorophenol + H2O substrate of mutant M379V, not of the wild-type enzyme Citrobacter freundii ?
-
?
o-cresol + H2O substrate of mutant M379V, not of the wild-type enzyme Citrobacter freundii ?
-
?
o-methoxyphenol + H2O substrate of mutant M379V, not of the wild-type enzyme Citrobacter freundii ?
-
?

Synonyms

Synonyms Comment Organism
tyrosine phenol lyase
-
Citrobacter freundii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 phenol as substrate Citrobacter freundii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Citrobacter freundii