Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Citrobacter freundii |
Protein Variants | Comment | Organism |
---|---|---|
F36V | changed substrate specificity | Citrobacter freundii |
F448V | changed substrate specificity | Citrobacter freundii |
M288V | changed substrate specificity | Citrobacter freundii |
M379V | changed substrate specificity | Citrobacter freundii |
M379V | site-directed mutagenesis, the mutant is active with o-cresol, o-methoxyphenol, and o-chlorophenol, as well as 3-methyl-, 3-methoxy-, 3 -fluoro, and 3-chloro-L-tyrosine in contrast to the wild-type enzyme | Citrobacter freundii |
T125S | changed substrate specificity | Citrobacter freundii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + H2O | Citrobacter freundii | - |
phenol + pyruvate + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chloro-L-tyrosine + H2O | substrate of mutant M379V, not of the wild-type enzyme | Citrobacter freundii | ? | - |
? | |
3-fluoro-L-tyrosine + H2O | substrate of mutant M379V, not of the wild-type enzyme | Citrobacter freundii | ? | - |
? | |
3-methoxy-L-tyrosine + H2O | substrate of mutant M379V, not of the wild-type enzyme | Citrobacter freundii | ? | - |
? | |
3-methyl-L-tyrosine + H2O | substrate of mutant M379V, not of the wild-type enzyme | Citrobacter freundii | ? | - |
? | |
L-tyrosine + H2O | - |
Citrobacter freundii | phenol + pyruvate + NH3 | - |
r | |
additional information | substrate specificity with 3-substituted tyrosines, the wild-type enzyme shows a narrow substrate spectrum, overview. Mutant M379V converts several L-tyrosine derivatives to the correspondent products | Citrobacter freundii | ? | - |
? | |
o-chlorophenol + H2O | substrate of mutant M379V, not of the wild-type enzyme | Citrobacter freundii | ? | - |
? | |
o-cresol + H2O | substrate of mutant M379V, not of the wild-type enzyme | Citrobacter freundii | ? | - |
? | |
o-methoxyphenol + H2O | substrate of mutant M379V, not of the wild-type enzyme | Citrobacter freundii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tyrosine phenol lyase | - |
Citrobacter freundii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | phenol as substrate | Citrobacter freundii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Citrobacter freundii |