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Literature summary for 4.1.99.2 extracted from

  • Milic, D.; Demidkina, T.V.; Faleev, N.G.; Matkovi?-Calogovi?, D.; Antson, A.A.
    Insights into the catalytic mechanism of tyrosine phenol-lyase from X-ray structures of quinonoid intermediates (2008), J. Biol. Chem., 283, 29206-29214.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the plasmid pTZTPL is constructed for expression of the protein in Escherichia coli SVS370 cells Citrobacter freundii

Crystallization (Commentary)

Crystallization (Comment) Organism
the quinonoid intermediates of tyrosine phenol-lyase with L-alanine and L-methionine are trapped in the crystalline state and their structures are determined at 1.9 A and 1.95 A resolution, respectively Citrobacter freundii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + H2O Citrobacter freundii
-
phenol + pyruvate + NH3
-
r

Organism

Organism UniProt Comment Textmining
Citrobacter freundii P31013
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Citrobacter freundii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + H2O
-
Citrobacter freundii phenol + pyruvate + NH3
-
r

Subunits

Subunits Comment Organism
homotetramer composed of two catalytic dimers Citrobacter freundii

Synonyms

Synonyms Comment Organism
TPL
-
Citrobacter freundii
tyrosine phenol-lyase
-
Citrobacter freundii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Citrobacter freundii