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Literature summary for 4.1.99.2 extracted from

  • Wierckx, N.J.; Ballerstedt, H.; de Bont, J.A.; de Winde, J.H.; Ruijssenaars, H.J.; Wery, J.
    Transcriptome analysis of a phenol-producing Pseudomonas putida S12 construct: genetic and physiological basis for improved production (2008), J. Bacteriol., 190, 2822-2830.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
Targeted gene disruptions are performed. Gene replacement vectors for the hpd, pobA, phhA, and aroP1 genes are created from pJQ200SK with different primers. Vectors are used to mutate the selected genes in Pseudomonas putida S12TPL3c by homologous recombination. Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
phenol In presence of 1 mM, only 23% of the original activity remained. The presence of phenol seriously affects its own production, through the inhibition of TPL Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + H2O Pseudomonas putida
-
phenol + pyruvate + NH3
-
r

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture wild-type S12 and its mutant strains S12TPL3 (optimized for phenol production) Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0022
-
in strain S12TPL3-delta-hpd mutant (4-hydroxyphenylpyruvate dioxygenase knockout), harvested in logarithmic phase Pseudomonas putida
0.0053
-
in strain S12TPL3, in presence of 1 mM phenol Pseudomonas putida
0.0078
-
in strain S12TPL3, harvested in logarithmic phase Pseudomonas putida
0.0232
-
in strain S12TPL3, without phenol Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + H2O
-
Pseudomonas putida phenol + pyruvate + NH3
-
r

Synonyms

Synonyms Comment Organism
TPL
-
Pseudomonas putida
tyrosine-phenol lyase
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas putida