Cloned (Comment) | Organism |
---|---|
Targeted gene disruptions are performed. Gene replacement vectors for the hpd, pobA, phhA, and aroP1 genes are created from pJQ200SK with different primers. Vectors are used to mutate the selected genes in Pseudomonas putida S12TPL3c by homologous recombination. | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenol | In presence of 1 mM, only 23% of the original activity remained. The presence of phenol seriously affects its own production, through the inhibition of TPL | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + H2O | Pseudomonas putida | - |
phenol + pyruvate + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | wild-type S12 and its mutant strains S12TPL3 (optimized for phenol production) | Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0022 | - |
in strain S12TPL3-delta-hpd mutant (4-hydroxyphenylpyruvate dioxygenase knockout), harvested in logarithmic phase | Pseudomonas putida |
0.0053 | - |
in strain S12TPL3, in presence of 1 mM phenol | Pseudomonas putida |
0.0078 | - |
in strain S12TPL3, harvested in logarithmic phase | Pseudomonas putida |
0.0232 | - |
in strain S12TPL3, without phenol | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + H2O | - |
Pseudomonas putida | phenol + pyruvate + NH3 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
TPL | - |
Pseudomonas putida |
tyrosine-phenol lyase | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |