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Literature summary for 4.1.99.2 extracted from

  • Kim, D.Y.; Rha, E.; Choi, S.L.; Song, J.J.; Hong, S.P.; Sung, M.H.; Lee, S.G.
    Development of bioreactor system for L-tyrosine synthesis using thermostable tyrosine phenol-lyase (2007), J. Microbiol. Biotechnol., 17, 116-122.
    View publication on PubMed
Search for more literature for 4.1.99.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 Symbiobacterium toebii

General Stability

General Stability Organism
the enzyme maintains its original activity at phenol concentrations below 75 mM Symbiobacterium toebii

Inhibitors

Inhibitors Comment Organism Structure
phenol 100 mM and above Symbiobacterium toebii

Organism

Organism UniProt Comment Textmining
Symbiobacterium toebii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by methanol treatment Symbiobacterium toebii

Storage Stability

Storage Stability Organism
40°C, in the presence of 75 mM phenol at pH 8.5, 18h, no loss of activity Symbiobacterium toebii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + H2O
-
 Symbiobacterium toebii phenol + pyruvate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
L-tyrosine phenol-lyase
-
 Symbiobacterium toebii
TPL
-
 Symbiobacterium toebii

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5
-
 Symbiobacterium toebii

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Symbiobacterium toebii